The Troponin I Serum/Whole Blood Rapid Test is a sandwich immunoassay. When sample is added to sample pad, it moves through the conjugate pad and mobilizes gold anti-cTnI conjugate that is coated on the conjugate pad. The mixture moves along the membrane by capillary action and reacts with anti-cTnI antibody that is coated on the test region. If cTnI is present at levels of 0.11 ng/mL ( Bayer ACS: 180 ) or 1.5 ng/mL ( Abbott AxSYM ) or greater* , the result is the formation of a colored band in the test region. If there is no cTnI in the sample, the area will remain colorless. The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the result is valid.
*The concentration of Troponin I is not standardized yet. The results from different assay system may vary significantly. Here is the summary of the sensitivity of Troponin I Test Card on the major assay systems.