c-di-GMP

Content
1 mg of lyophilized c-di-GMP Vaccine grade adjuvant.
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Storage and Stability
c-di-GMP is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
Upon resuspension, prepare aliquots of c-di-GMP and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.

Description
Cyclic diadenylate monophosphate (c-di-GMP) is an intracellular signaling molecule produced by bacteria. Administration of c-di-GMP can induce a strong immune response in vitro and in vivo. It was recently found that this
cyclic dinucleotide induces the production of cytokines, such as type I interferons, through the STING/TBK1/IRF3
pathway. Due to its immunostimulatory properties, this molecule has been investigated as a vaccine adjuvant. Mucosal delivery of c-di-GMP elicits a balanced Th1/Th2 profile and Th17 response2, which is crucial against intracellular pathogens. This adjuvant acts through the recruitment of monocytes and granulocytes, and the maturation of dendritic cells.

Preparation of Sterile Stock Solution (2 mg/ml)
Add 500 μl endotoxin-free physiological water to the 1 mg c-di-GMP vial to obtain a solution at 2 mg/ml.
Mix the solution by pipetting up and down.

Vaccine Grade