Bovine Sterile Filtered Serum (DAGC281)

Product Overview
The raw materials for these products were collected from cattle 30 months and younger in the USA in abattoirs registered with the USDA. The animals received ante and post-mortem inspections under a veterinarian's supervision and were apparently free from infectious and contagious diseases. At no time during collection or processing was the material commingled with any other material of animal origin.
Target
Bovine Serum
Nature
Native
Tag/Conjugate
Unconjugated
Alternative Names
Bovine Serum; Serum
Procedure
None
Purity
0.2 micron (Absolute)
Format
Liquid
Concentration
Hemoglobin: <30 mg/dl
Protein:>6.0 g/dl
Size
100ml, 500ml
Buffer
pH: 6.8 - 8.2
Preservative
None
Storage
This serum is stable for ≥ 5 years when stored −20°C ± 5°C. Avoid repeated freezing and thawing.
Keywords
Bovine Serum; Serum

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References


Effect of [EMIM][BF4] ionic liquid on the properties of ultrafiltration membranes

POLYMER

Authors: Lessan, Fatemeh; Foudazi, Reza

We employ a miscible mixture of an imidazolium based ionic liquid (IL) and dimethylformamide (DMF) as a good solvent of polyethersulfone (PES) for fabrication of membranes by nonsolvent induced phase separation (NIPS) method. The addition of IL to the cast solution changes solvent quality, which leads to variation of rheological behavior of polymer solution as well as developed membranes performance. We characterize the prepared membranes in terms of water flux, bovine serum albumin (BSA) rejection, pore size, porosity, and water contact angle. Results show that IL influences the kinetics and thermodynamic of phase separation of PES solution. Viscosity values as well as thermodynamic instability of PES solution increase with IL concentration, which lead to higher porosity and pore size of final membranes. Consequently, 17 wt% IL in formulation leads to an increase of mean pore size from 2.0 to 12.7 nm which improves water flux of final membranes from 0.5 to 77 kg/m(2)h. The results of fouling measurements show that by increasing IL content, the reversible fouling increases. Additionally, the irreversible fouling ratio reduces from 0.42 to 0.28 for 0 and 17 wt% IL containing formulations, respectively. The newly developed membranes provide 97% rejection of BSA solution and can be used as ultrafiltration membranes for the separation and concentration of macromolecules and proteins.

Novel electrochemical biosensing platform for microRNA detection based on G-quadruplex formation in nanochannels

SENSORS AND ACTUATORS B-CHEMICAL

Authors: Zhao, Fei; Zhang, Hongfang; Zheng, Jianbin

Ultrasensitive detection of microRNA is crucial important in the diagnosis of related diseases. By immobilizing the capture probes in nanochannels, we developed a label-free electrochemical biosensing platform based on guanine-quadruplex (G-quadruplex) formation in nanochannels. The designed capture probe contains both the microRNA-21 (MiR-21) recognition sequence and guanine-rich sequence. The presence of the target MiR-21 would trigger the enzymatic cleaving of the RNA/DNA heteroduplexes which led some of the guanine-rich sequences escape from the nanochannels and thus reduced the formation of the G-quadruplex/hemin complex. Therefore, the quantity of methylene blue fluxed through the nanochannels changed because of the variation of the steric hindrance. A optimized carbon nanofibers modified electrode was applied to monitor the quantity difference of methylene blue. In this way, the biosensing platform realized the label-free electrochemical detection of MiR-21 with a ultralow detection limit of 0.5 aM. Meanwhile, this biosensing platform was assessed for MiR-21 detection in serum samples. The biosensing platform can be readily extended to the assay of other scarcetumor-related genes.

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