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The boldenone ELISA Test Kit is a professional in vitro diagnostic (or research-use) tool designed for high-sensitivity screening of boldenone, which is an anabolic steroid widely used illegally in sports, livestock breeding, and other fields. Based on the Enzyme-Linked Immunosorbent Assay (ELISA) principle, this kit enables rapid, accurate, and cost-effective qualitative or quantitative detection of boldenone residues in various samples (such as serum, urine, tissue homogenates, and feed extracts). It is widely applicable to sports anti-doping laboratories, food safety supervision institutions, veterinary inspection centers, and scientific research institutions, providing reliable technical support for controlling Boldenone abuse and ensuring public health and sports fairness.
Boldenone, also known as 1,4-androstadien-17β-ol-3-one, is a synthetic anabolic steroid with strong muscle-building and growth-promoting effects. In sports, its illegal use can enhance athletic performance but causes serious health risks (e.g., liver damage, cardiovascular diseases, and hormonal disorders), violating the principles of fair competition. In livestock breeding, unauthorized addition of Boldenone to feed leads to residues in animal-derived foods (meat, milk, eggs), which may cause endocrine disruption and other health hazards when ingested by humans. Traditional detection methods for boldenone (such as liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS)) have high accuracy but require expensive equipment, professional operators, and long detection cycles—making them unsuitable for large-scale rapid screening.
Figure 1. Boldenone.
Sample Compatibility
Sensitivity Range
Main Uses
Feature/Specification
Intended Use
Product Features
Three Core Advantages
| Parameter | Details |
| Product name | Boldenone ELISA Kit |
| Sample | Urine, Blood, Oral Fluid, Forensic Matrices |
| General Description | CD's Boldenone ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS). |
| Storage | This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C. Store controls frozen if not used within 10 days. Note: Some kits require controls to be stored frozen immediately upon receipt. Reference kit label for details. |
Compared with other boldenone detection methods or similar ELISA kits, this product has obvious competitive advantages:
More flexible sample adaptability: Some kits only support a single sample type. This kit, through optimized buffer systems, can handle serum, tissue, and feed samples with simple pre-treatment, reducing the need for multiple kit purchases.
Cost-effectiveness: Compared with LC-MS/GC-MS, the kit has lower equipment investment and per-sample detection costs, suitable for long-term routine screening.
Complete Supporting Reagents: The kit provides all necessary reagents (including standards and quality controls), avoiding the trouble of purchasing and matching reagents separately.
Operational Convenience Advantage
The kit simplifies complex detection processes (e.g., no need for professional instrument operation or complex sample purification). With a detailed instruction manual, even personnel without advanced laboratory experience can complete detection after simple training, reducing the threshold for use.
Reliability and Compatibility Advantage
Under strict quality control, the kit's sensitivity, specificity, and precision meet international technical standards; it is compatible with common microplate readers on the market (no need for dedicated equipment), ensuring seamless integration with existing laboratory workflows.
High Specificity for Accurate Clinical Assessment
The antibody used in this kit exhibits high affinity and specificity for progesterone, minimizing interference from other structurally similar steroid hormones. This ensures the accuracy and reliability of results, which is paramount for fertility assessment and pregnancy monitoring.
The boldenone ELISA kit, with its high sensitivity, strong specificity, simple operation, and cost-effectiveness, effectively addresses the pain points of large-scale, rapid screening for boldenone. It not only provides a reliable technical tool for sports anti-doping (maintaining fair competition) and food safety supervision (protecting public health) but also supports scientific research in pharmacology and toxicology. Whether in government supervision institutions, third-party testing laboratories, or scientific research units, this kit can meet diverse detection needs and is an ideal choice for boldenone screening.
A1: It's not possible. The ELISA method is commonly used for initial screening detection. For positive samples, it is recommended to use confirmation methods such as GC-MS or LC-MS/MS for final confirmation and quantification.
A2: Usually requires simple dilution and centrifugation. Please refer to the product manual for specific sample pre-processing methods, as different sample types may have different processing methods.
A3: This reagent kit is mainly designed for detecting the prototype of benzophenone. But its antibodies may have some cross reactivity with certain major metabolites, thus achieving indirect monitoring. To specifically detect a certain metabolite, it is necessary to confirm the cross reaction data of the kit.
A4: A 96 well plate reagent kit, with standard curves and quality control wells set, can typically detect about 40 unknown samples (each sample is double well).
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