Biotinylated Human DLL4 ELISA Kit

Sample

Cell supernatant, serum and plasma

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

The Biotinylated Human DLL4 ELISA Kit was developed for the detection and quantitative determination of GMP human Biotinylated DLL4 in samples from CAR-T product preparation processing. It is intended for research use only (RUO).

Contents of Kit

1. Pre-coated Anti-DLL4 Antibody Microplate: 1 plate, store at 2-8°C once opened
2. Biotinylated Human DLL4 Standard (Lyophilized): 20 μg, store at -70°C once opened
3. Streptavidin-HRP: 50 μL, store at 2-8°C (avoid light) once opened
4. 10× Washing Buffer: 50 mL, store at 2-8°C once opened
5. 2× Dilution Buffer: 50 mL, store at 2-8°C once opened
6. Substrate Solution: 12 mL, store at 2-8°C (avoid light) once opened
7. Stop Solution: 7 mL, store at 2-8°C once opened

Storage

1. Unopened kit should be stored at 2°C-8°C upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per Reagents And Materials Provided. The shelf life is 30 days from the date of opening.

Precision

Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision: Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

Sensitivity

The minimum detectable concentration of human Biotinylated DLL4 is 0.771 pg/mL. The minimum detectable concentration was determined by adding twice standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

General Description

Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activate NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4. To support the development of CAR-T drugs, Creative Diagnostics independently developed human Biotinylated DLL4 ELISA Kit via rigorous methodological validation, which is used for detection of GMP human Biotinylated DLL4 in samples from CAR-T product preparation processing for evaluation the quality of CAR-T products in drug development and CMC quality control stages.

Standard Curve