RHA™ anti-biotin monoclonal antibody, clone BI (HMABPY074)

RHA™ mouse anti-biotin monoclonal antibody, clone BI for ELISA, LFIA


Host Species
Antibody Isotype
Species Reactivity
Biotin [KLH]


Application Notes
Indirect ELISA IC50: 25 ppb
Matched pair conjugate available for Biotin antibody:
Biotin [BSA(Catalog # DAG3197)
Biotin [HRP](Catalog # DAG3197-HRP)
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.


Alternative Names
Biotin; D-biotin; Vitamin H


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Custom Antibody Labeling

We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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Rapid conjugation of antibodies to toxins to select candidates for the development of anticancer Antibody-Drug Conjugates (ADCs)


Authors: Hoffmann, Ricarda M.; Mele, Silvia; Cheung, Anthony; Larcombe-Young, Daniel; Bucaite, Gintare; Sachouli, Eirini; Zlatareva, Iva; Morad, Hassan O. J.; Marlow, Rebecca; McDonnell, James M.; Figini, Mariangela; Lacy, Katie E.; Tutt, Andrew J. N.; Spicer, James F.; Thurston, David E.; Karagiannis, Sophia N.; Crescioli, Silvia

Antibody-Drug Conjugates (ADCs) developed as a targeted treatment approach to deliver toxins directly to cancer cells are one of the fastest growing classes of oncology therapeutics, with eight ADCs and two immunotoxins approved for clinical use. However, selection of an optimum target and payload combination, to achieve maximal therapeutic efficacy without excessive toxicity, presents a significant challenge. We have developed a platform to facilitate rapid and cost-effective screening of antibody and toxin combinations for activity and safety, based on streptavidin-biotin conjugation. For antibody selection, we evaluated internalization by target cells using streptavidin-linked antibodies conjugated to biotinylated saporin, a toxin unable to cross cell membranes. For payload selection, we biotinylated toxins and conjugated them to antibodies linked to streptavidin to evaluate antitumour activity and pre-clinical safety. As proof of principle, we compared trastuzumab conjugated to emtansine via streptavidin-biotin (Trastuzumab-SB-DM1) to the clinically approved trastuzumab emtansine (T-DM1). We showed comparable potency in reduction of breast cancer cell survival in vitro and in growth restriction of orthotopic breast cancer xenografts in vivo. Our findings indicate efficient generation of functionally active ADCs. This approach can facilitate the study of antibody and payload combinations for selection of promising candidates for future ADC development.

A biotin-guided hydrogen sulfide fluorescent probe and its application in living cell imaging


Authors: Zhang, Chen; Zhang, Jiewen; Xu, Zhiqiang; Zang, Kun; Liu, Feng; Yin, Jun; Tan, Ying; Jiang, Yuyang

Hydrogen sulfide (H2S), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of H2S offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent probe, NP-biotin, was developed for H2S detection and evaluated in normal liver cell (LO2) and liver cancer cell (HepG2) lines. Results reveal that NP-biotin can detect cellular H2S with high sensitivity and selectivity. Moreover, NP-biotin has been confirmed to possess the ability to target cancer cells under the guidance of the biotin group.

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