Q: I was interested in any further details concerning DMAB-CBXY25039, particularly regarding cross-reactivity with other allergens or any known background reactivity.
A: This antibody exhibits exceptional specificity for Bet v 1, with no cross-reactivity to common allergens (Phl p 1, Der p 1, Fel d 1) or BSA.
Defined Epitope: The antibody targets a specific conformational epitope within residues I56 to D69 of Bet v 1.0101, located in the flexible loop connecting β-strands 3 and 4.
Epitope Precision: Binding is critically dependent on residues P59, F62, P63, and K65. Notably, the F62S mutation (found in Mal d 1.0108 and Aln g 1.0101) results in a total loss of recognition, enabling precise discrimination between closely related homologues.
Isoform Selectivity: It specifically recognizes natural isoforms Bet v 1.0101 and 1.0102, while showing no reactivity with isoform 1.0112 or engineered hypoallergenic variants (e.g., Bet v 1.2744, 1.2595).
Superior Performance: Demonstrates limited cross-reactivity across the PR-10 family, ensuring exceptionally high signal-to-noise ratios in complex biological assays.
COA
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