Human Anti-Human B2GPI monoclonal antibody, clone G

Antibody Isotype

IgG

Clone

G

Species Reactivity

Human

Conjugate

Application Notes

ELISA
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

General Notes

CABT-L6309 exhibits strong reactivity with immobilized β2GPI in ELISA, with a detection limit as low as 20 ng/ml, but only modest interaction with soluble β2GPI in the fluid phase. This characteristic is not a drawback for standard indirect ELISA (where antigen is pre-coated) and may even reduce interference from free antigen; however, assay formats using solution-phase capture may require optimization.
CABT-L6309 specifically recognizes an epitope in Domain I (DI) of human β2GPI, which is the primary target of pathogenic autoantibodies in Antiphospholipid Syndrome (APS). Moreover, when β2GPI is bound to cardiolipin, CABT-L6309 binds not only to human and mouse β2GPI but also to β2GPI from rat, pig, goat, and fetal calf, with varying degrees of reactivity.