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Aldosterone (ALD) and Dihydrotestosterone (DHT) are two critical steroid hormones governing essential physiological processes, from blood pressure regulation and electrolyte balance to male sexual development and metabolic function. The precise quantification of these hormones is paramount in both basic scientific inquiry and clinical investigation. High-sensitivity ALD(Aldosterone) ELISA (Enzyme-Linked Immunosorbent Assay) Kits provide researchers with robust, reliable, and user-friendly tools to accurately measure these biomarkers in a variety of biological samples, thereby unlocking insights into endocrine, cardiovascular, urological, and metabolic diseases.
Aldosterone (ALD)
Aldosterone is secreted by the zona glomerulosa of the adrenal cortex and is the core effector hormone of the renin angiotensin aldosterone system (RAAS). Its main physiological function is to promote the reabsorption of sodium ions and excretion of potassium ions in the renal distal tubules and collecting ducts, thereby maintaining fluid capacity and electrolyte balance. Abnormal aldosterone levels are closely related to various diseases:
Aldosteronism: including primary aldosteronism (such as Conn syndrome, which is an important cause of refractory hypertension) and secondary aldosteronism (such as renal artery stenosis, heart failure, etc.).
Aldosterone deficiency: commonly seen in adrenal cortex dysfunction (such as Addison's disease).
Therefore, accurate detection of ALD concentration is crucial for the etiological classification of hypertension, assessment of electrolyte imbalances, and judgment of adrenal function.
Dihydrotestosterone (DHT)
Dihydrotestosterone is a potent androgen that is converted from testosterone by 5 α - reductase catalysis, and its androgenic activity is about 2-3 times that of testosterone. DHT plays a central role in male embryonic sexual differentiation, development of secondary sexual characteristics during puberty, and prostate growth in adulthood. Abnormal levels of DHT can also lead to a series of pathological states:
High levels of DHT: an important pathogenic factor for benign prostatic hyperplasia (BPH) and prostate cancer. In addition, it is directly associated with male androgenetic alopecia (seborrheic alopecia).
5 α - reductase deficiency: a rare genetic disease in which patients suffer from developmental abnormalities such as male pseudohermaphroditism due to DHT synthesis disorders.
In clinical and scientific research, quantitative detection of DHT has extremely high value for the diagnosis and monitoring of prostate diseases, the study of hair loss mechanisms, and the diagnosis of sexual development disorders.
Enzyme linked immunosorbent assay (ELISA) is a highly sensitive and specific immunoassay technique, particularly suitable for quantitative detection of low concentration substances in complex biological samples such as serum, plasma, urine, and tissue homogenates. The core principle is to utilize the specific binding properties of antigens and antibodies, and amplify this binding signal through enzymatic reactions, converting it into a measurable optical signal (absorbance value).
Detection principle of ALD and DHT ELISA kits (competitive method)
Due to the lack of multiple antigenic epitopes in small molecule steroid hormones such as ALD and DHT, competitive ELISA is commonly used. The brief process is as follows:
Coating: The pore walls of the microplate are pre coated with capture antibodies (targeting DHT or ALD antibodies) or conjugates similar in structure to the target hormone.
Competitive response: Simultaneously adding the test sample (containing unknown concentration of target hormone) and a certain amount of enzyme labeled target hormone (enzyme labeled antigen). Natural hormones and enzyme-linked antigens in the sample will competitively bind to limited antibody binding sites.
Washing: Wash away unbound components, leaving only the complex bound to the antibody in the well.
Color development: Adding enzyme substrate (such as TMB) produces a blue product under the action of binding enzyme.
Termination and measurement: Add termination solution to terminate the reaction, and the solution turns yellow. Use an enzyme-linked immunosorbent assay (ELISA) reader to measure the absorbance (OD value) of each well at a wavelength of 450nm.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Application of ALD ELISA Kit
Application of Dihydrotestosterone ELISA Kit
Key Features of the Product
| Parameter | Details |
| Product name | ALD(Aldosterone) ELISA Kit |
| Sample | serum, plasma, tissue homogenates, other biological fluids |
| Intended Use | ALD ELISA Kit allows for the in vitro quantitative determination of ALD concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Storage | Store the kit reagents at 2-8°C. For more detailed information, please download the following document on our website. |
Evaluating the efficacy of drugs targeting ALD (e.g., aldosterone antagonists like spironolactone) in preclinical models of hypertension or heart failure.
Assessing the impact of androgen-modulating drugs (e.g., 5α-reductase inhibitors, which reduce DHT synthesis) on DHT levels in preclinical studies.
Detecting the effects of environmental toxins or endocrine disruptors on ALD or DHT secretion in animal models.
Time and Cost Efficiency
The 96-well microplate format allows for the simultaneous analysis of up to 40 samples in duplicate per kit, significantly reducing per-sample cost and laboratory man-hours compared to single-analyte methods.
Broad Application Flexibility
Validated for use with multiple sample types, providing flexibility in experimental design, from in vitro cell studies to in vivo animal model analysis.
Superior Data Quality
The combination of high specificity and reproducibility minimizes background noise and data variability, leading to clearer, more publishable results.
In the pursuit of scientific discovery, the quality of your tools directly impacts the quality of your findings. Our Aldosterone and Dihydrotestosterone ELISA Kits are engineered to the highest standards of sensitivity, specificity, and reliability, empowering you to generate robust data with confidence. Whether you are exploring the complexities of the RAAS system or deciphering androgen signaling pathways, these kits provide the precision necessary to drive your research forward.
A1: It depends on the sample type and expected hormone concentration. For most serum and plasma samples, our kits are designed for direct use. However, for urine or samples with very low concentrations, extraction may be recommended to concentrate the analyte and remove interfering substances. Please refer to the specific kit manual for detailed guidance.
A2: Sandwich ELISAs are used for large molecules with multiple antigenic sites (like cytokines), using two antibodies for capture and detection. Competitive ELISAs are ideal for small molecules like ALD and DHT, which have a single epitope. In a competitive format, the analyte in the sample competes with a labeled analyte for a limited number of antibody binding sites.
A3: Each kit contains one 96-well plate. Considering the standards and controls, a single kit typically allows for the analysis of approximately 40 unknown samples in duplicate.
A4: No. These kits are labeled "For Research Use Only" and are not intended for use in diagnostic procedures. They are not FDA-approved for clinical diagnosis.
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