Rabbit Anti-Human WASP Polyclonal Antibody Knockout Validated

Tenebrio molitor larvae protein isolates (TPIs) were extracted using the alkaline extraction and acid precipitation methods (AEAP) assisted by NaCl (salting-in) and (NH4)(2)SO4 (salting-out) procedures. The structural, physicochemical, and functional properties of TPIs were investigated. It was found that the salt-assisted treatments did not affect the total amino acid content but altered specific amino acid compositions. The salting-in-AEAP extraction resulted in non-significant (P > 0.05) differences in zeta potential, hydrophobicity, thermal stability, solubility and foaming capacity compared with the AEAP extraction. Salting-out-AEAP extraction significantly (P < 0.05) increased overall protein solubility, emulsion activity, foaming capacity and stability that were associated with lower hydrophobicity, higher zeta potential, alpha-helix and disulfide bond contents. The salting-inAEAP-out extraction generated the greatest protein yield (39.54%), emulsion activity index (55.5 m(2)/g), foaming capacity (205%) as well as foaming stability (65.59%).

Peppermint is widely used medicinal plant with distinguished bioactive potential, therefore, the aim of present work was to develop novel peppermint extracts with high activity by application of traditional and emerging separation techniques. Conventional hydrodistillation and microwave-assisted hydrodistillation (MWHD) were applied for recovery of essential oil (EO), while organic solvent extraction using Soxhlet apparatus, microwave-assisted and ultrasound-assisted process and supercritical fluid extraction (SFE) were applied for non-selective recovery of peppermint lipophilic extracts. Extracts were characterized in terms of terpenoids profile with special emphasis on content of major compounds (mentol, menthone, isomenthol and eucalyptol). Antioxidant activity (DPPH, ABTS, CUPRAC, FRAP, chelating and phosphomolybdenum assay) and enzyme-inhibitory assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase and glucosidase inhibition) were used for screening of peppermint bioactivity. MWHD was recognized as alternative for traditional process in EO recovery, while SFE extracts were useful for green production of solvent-free peppermint extracts rich in terpenoids and other lipophilic bioactives.