Anti-SFN Polyclonal Antibody (CABT-L2218) Knockout Validated

Rabbit Anti-Human SFN (Met1~Ser248) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Rat
Immunogen
Recombinant fragment corresponding to human SFN (Met1~Ser248)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.2-2 ug/ml; 1:250-2500
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type Hela cell lysate. Lane 2: SFN knockout Hela cell lysate. Predicted MW: 28kd, Observed MW: 30kd
Western Blot. Sample. Human A431 cell lysate.
Western Blot. Sample. Human Hela cell lysate.

Target


Alternative Names
YWHAS; HME1; 14-3-3 Protein Sigma; Epithelial cell marker protein 1
Entrez Gene ID
UniProt ID

Product Background


Pathway
Activation of BAD and translocation to mitochondria; Activation of BH3-only proteins; Aldosterone-regulated sodium reabsorption; Alpha6-Beta4 Integrin Signaling Pathway; Apoptosis; Calcium Regulation in the Cardiac Cell; Cell cycle; Class I PI3K signaling events mediated by Akt;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Pasqualini, R; Bourdoulous, S; et al. A polymeric form of fibronectin has antimetastatic effects against multiple tumor types. NATURE MEDICINE 2:1197-1203(1996).
Lu, QX; Xin, Y; et al. 14-3-3 sigma Controls Corneal Epithelium Homeostasis and Wound Healing. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 52:2389-2396(2011).

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