Anti-NR3C1 Polyclonal Antibody (CABT-L2141) Knockout Validated

Rabbit Anti-Human NR3C1 (Leu532~Glu730) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Recombinant fragment corresponding to human NR3C1 (Leu532~Glu730)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.2-2 ug/ml; 1:250-2500
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type HepG2 cell lysate. Lane 2: NR3C1 knockout HepG2 cell lysate. Predicted MW: 78~85, 60~64kd, Observed MW: 97kd
Western Blot. Sample. Human A431 cell lysate.
Western Blot. Sample. Human Hela cell lysate.
Western Blot. Sample. Human HepG2 cell lysate.

Target


Alternative Names
GR; GCCR; GCR; GR; GRL; NR3-C1
Entrez Gene ID
UniProt ID

Product Background


Pathway
Adipogenesis; BMAL1:CLOCK,NPAS2 activates circadian gene expression; Circadian Clock; FOXA2 and FOXA3 transcription factor networks; Gene Expression; Generic Transcription Pathway; Glucocorticoid receptor regulatory network; Neuroactive ligand-receptor interaction;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Kolla, V; Robertson, NM; et al. Identification of a mineralocorticoid/glucocorticoid response element in the human Na/K ATPase alpha 1 gene promoter. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 266:5-14(1999).

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