Anti-Interferon beta Binding Antibodies ELISA Kit

Sample

Serum, plasma and other biological fluids.

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-IFNb in human serum, plasma and other biological fluids.

Contents of Kit

Pre-coated, ready to use 96-well strip plate 1
Standard 2 vials
Standard Diluent 1×20mL
Detection Reagent A 1×120μL
Assay Diluent A 1×12mL
TMB Substrate 1×9mL
Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL
Plate sealer for 96 wells 4
Instruction manual 1

Storage

1. For unused kit: The whole kit could be stored at -20°C in shelf life, while up to one month at 4°C. For experiment convenience, reagents could also be stored seperately, Standard, Detection Reagent A and 96-well strip plate should be stored at -20°C while the others could be at 4°C.
2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note:
It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. For the expiration date of the kit, please refer to the label on the kit box. All components are stable until this expiration date.

Performance Characteristics

STABILITY
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially roomtemperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that thewhole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-IFNb were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-IFNb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/mean×100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Detection Range

3.12-200ng/mL. The standard curve concentrations used for the ELISA's were 200ng/mL, 100ng/mL, 50ng/mL,25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL.

Sensitivity

The minimum detectable dose of Anti-IFNb is typically less than 1.19ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentrationthat could be differentiated from zero. It was determined by adding two standard deviations to the mean opticaldensity value of twenty zero standard replicates and calculating the corresponding concentration.

Standard Curve

In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the knownconcentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is thedependent variable. However, the O.D. values of the standard curve may vary according to the conditions ofassay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log ofthe data to establish standard curve for each test is recommended. Typical standard curve below is provided forreference only.