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Androstenedione ELISA kit is a powerful and highly specific tool designed for quantitative detection of androstenediol in human serum, plasma, and other biological fluids. This kit adopts a sensitive and validated competitive enzyme-linked immunosorbent assay (ELISA) format, providing researchers and clinicians with a reliable method to obtain accurate data necessary for evaluating adrenal and gonadal function. Its optimized scheme ensures excellent repeatability, making it an indispensable asset in endocrinology, reproductive medicine, and endocrine disease research.
Androstenedione is an important steroid hormone produced by the adrenal gland and gonads (ovaries and testes). It is a direct precursor of testosterone and estrone and plays a crucial role in the biosynthesis pathway of sex hormones. Accurately measuring levels of androstenedione is crucial for diagnosing and managing various endocrine disorders. It is particularly important in evaluating female hyperandrogenism (such as polycystic ovary syndrome - PCOS), diagnosing congenital adrenal hyperplasia (CAH), and assessing adrenal tumors or function. Its level is also a sign of interest in anti doping research.
This ELISA kit is based on the principle of competition. The endogenous androstenedione in the sample competes with androstenedione enzyme conjugates (such as horseradish peroxidase) to obtain binding sites on a limited amount of androstenedione specific antibodies pre coated on a microplate. After the incubation and washing steps to remove unbound substances, the substrate solution is added to generate a colorimetric signal. The intensity of this signal is inversely proportional to the concentration of androstenedione in the sample, allowing for accurate quantification.
Sample Compatibility
Sensitivity Range
Main Uses
Intended Use
Product Specifications
Key Features of the Product
Performance
| Parameter | Details |
| Product name | Androstenedione ELISA Kit |
| Sample | Androstenedione serum, EDTA plasma |
| Intended Use | The Androstenedione ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Androstenedione in serum and EDTA plasma. |
| Storage | Store the kit at 2-8°C. Keep microwells sealed in a dry bag with desiccants. For more detailed information, please download the following document on our website. |
Critical Role in Hormonal Pathway Analysis
This kit provides a direct measurement method for key intermediate hormones. Compared to measuring testosterone alone, quantifying androstenedione can provide a more specific understanding of the sources of androgen excess (adrenal and ovarian), which is highly valuable for differential diagnosis in endocrine research.
High Specificity for Accurate Assessment
The antibodies used in this kit exhibit high affinity and specificity for androstenedione, minimizing interference from other structurally similar steroid hormones present in the serum. This ensures the accuracy of the results, which is crucial for explaining complex endocrine features.
Streamlined and Robust Workflow
The competitive ELISA format provides a simple and time-saving solution. By using pre coated plates and optimized reagents, measurements can be quickly completed, providing robust and reproducible results suitable for clinical and large-scale studies.
The androstenedione ELISA kit is a powerful tool for precise quantification of androstenediones, meeting the key needs of researchers and preclinical teams: high sensitivity, specificity, and ease of use. Its powerful performance, wide sample compatibility, and cost-effectiveness make it a superior alternative to traditional detection methods (RIA, GC-MS) in most endocrine research and drug applications. Whether studying hormone imbalances in diseases such as PCOS, monitoring drug effects in preclinical models, or conducting high-throughput screening, this kit can provide reliable results and accelerate scientific discoveries. With comprehensive quality control and specialized technical support, it is the ideal choice for anyone seeking to quantify this critical hormone precursor.
A1: Elevated levels of androstenedione are a common finding in polycystic ovary syndrome and a major cause of clinical hyperandrogenism, such as hirsutism. Its measurement can help confirm the diagnosis and evaluate the severity of androgen excess, which typically originates from the ovaries.
A2: Androstenedione is a direct precursor of testosterone. It is a weaker androgen, but its measurement can sometimes provide more sensitive indicators of adrenal androgen production. In some cases, such as CAH, testosterone levels may increase while testosterone remains normal.
A3: After receiving the reagent kit, it should be stored at a temperature of 2-8 ° C. Under these conditions, all ingredients remain stable before the expiration date printed on the packaging box. Unless otherwise specified in the manual, avoid freezing reagents.
A4: This kit has been specifically validated for human androstenedione. The cross reactivity of androstenediones with other species may differ and has not been validated. For animal research, it is recommended to use species-specific ELISA kits.
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