The Amb a 1 Antigen ELISA utilizes the enzyme-linked immunosorbent assay (ELISA) principle, where microtiter plates coated with antibodies specific to Amb a 1 capture the antigen from the sample. After binding, a secondary enzyme-linked antibody is added, which attaches to the antigen to form a complex. Following a washing step to remove unbound materials, a substrate solution is introduced that reacts with the enzyme, resulting in a color change. The intensity of this color change, measured using a spectrophotometer, correlates with the concentration of Amb a 1 in the sample, allowing for quantitative analysis.
Summary
Sample
Environment sample
Detection Method
sELISA
Intended Use
The Amb a 1 Antigen ELISA kit is intended for the quantitative detection of Amb a 1 antigen in various samples. This assay is designed for research purposes to aid in the identification of allergic reactions associated with Ambrosia pollen exposure, typically leading to allergic rhinitis and asthma symptoms.
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