Allergen-Specific Immunoglobulin E (IgE) Testing

Creative Diagnostics scientists are experienced in allergen-specific immunoglobulin E (IgE) Testing. Our testing capabilities include traditional to innovative testing technology and we have a powerful proprietary IgE Testing method for allergen specific immunoglobulin E (IgE) Testing from serum for early discovery studies, preclinical studies and diagnostic kit development. Each allergen-specific IgE test result is again and again confirmed as valid and genuine.

What is Allergen-Specific Immunoglobulin E?

The IgE is called allergen-specific immunoglobulin E (sIgE). B cells secrete it, it's a subtype of IgE, and most often is found in serum. Humans come in contact with allergens (eg, pollen, dust mites, food ingredient), and the immune system recognises these allergens and makes certain antibodies called IgE.

sIgE cleaves to the FcRI receptor on mast cells and basophils by its Fc segment. Allegens returned to the body that attached themselves to sIgE on the mast cells and basophils degranulate, exchanging mediators of inflammation like histamine and producing allergic symptoms. The reaction generally looks like sneezing, runny nose and itchiness. This sIgE expression is linked to many different cytokines and signalling pathways including IL-25, IL-33 and TSLP. Furthermore, Th2-type helper T cell response induces sIgE as well. It is often the sIgE level that is used to diagnose allergic illnesses and inform treatment decisions. sIgE is the basis antibody in allergies. It identifies and binds to allergens, and in so doing overreacts the immune system, resulting in many of the allergic symptoms.

Allergen-Specific IgE Test

IgE allergy test for testing allergy of the patient against the particular substance, detection of antibodies to certain allergens in a patient's blood. It is the most significant test for allergic disease diagnosis, treatment programme, and immune therapy evaluation. So, for instance, if a patient develops urticaria, dermatitis, asthma and so on, one could check the serum IgE to see if they're caused by a certain allergen.

Allergen-Specific IgE Enzyme-Linked Immunosorbent Assay (ELISA)

Enzyme-Linked Immunosorbent Assay (ELISA) is the most popular allergen-specific IgE detection method. It is based on using enzyme-labeled secondary antibodies to bind with specific IgE in the patient's serum, with the radioactive signal detected by a counter. 

Advantages of ELSIA testing for Allergen-Specific IgE

• High sensitivity
• Easy to perform
• Stable and reliable results
• No use of radioactive materials

Sample Types:

• Serum
• Plasma
• Whole Blood

In addition, allergen-specific IgE can also be detected by radioallergosorbent test (RAST), western blot, and fluorescent enzyme immunoassay (FEIA).

The Newest IgE Detection Technology for Allergens

1. Microarray: Microarray is one of the most promising technology for allergen-specific IgE detection in recent years. It employs the principle of molecular hybridisation to "print" many known DNA or protein probes onto a glass slide or chip, so that hundreds of allergen proteins can be detected at one time. This technology is also highly throughput, low sample quantity and parallel detection, and is ideally suited for diagnosing allergy in children.
2. ALEX2 4th generation immunoglobulin E (IgE) allergy test: ALEX2 4th generation IgE allergy test is the latest allergen-specific IgE technology. It uses high-resolution nanotechnology and molecular allergen detection to pick up to 300 whole allergens and molecular allergen combinations. It increases the detectability and decreases false positives through the elimination of cross-reaction (CCD) technology and gives you more accurate test results.
3. ImmunoCAP system: The ImmunoCAP is a sandwich detection technology that makes all the applicable antibodies bound using optimized cellulose particles. Not only does this system enhance test sensitivity and specificity, but clinical laboratories can perform entirely quantitative measurements and reproducibility.
4. Recombinant allergen proteins: In recent years, IgE testing for allergens has been more precise thanks to genetic recombination. The peanut proteins h2A9 and h3A9, for instance, are specific IgE markers in peanut allergies, and they're more sensitive and specific than conventional peanut extracts. But these recombinant protein parts can be less sensitive in some circumstances.

Creative Diagnostics offers Allergen-Specific IgE Testing Services

• ELISA Testing Services for sIgE
• RAST Testing Services for sIgE
• Western Blot Testing Services for sIgE
• FEIA Testing Services for sIgE
• Microarray Technology Testing Services for sIgE
• ALEX2 Testing Services for sIgE
• ImmunoCAP System Testing Services for sIgE

How it works:

The creative diagnostics Allergen-specific immunoglobulin E (IgE) Testing process is easy. Tell us some basic information such as:

• The method you want to test.
• The list of allergens you are interested in testing.

One of our consultants will get back to you to verify everything. After a quote is approved, just send your sample to our lab and test result report will reach your lab in time.