Specificity: Specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH).
Species Reactivity: Human, Mouse, Rat
Application: ELISA, FC, IF, IHC
Summary
Specifications
Antibody Isotype
IgG1k
Clone
3G7
Species Reactivity
Human; Mouse; Rat
Immunogen
ACTH synthetic peptide (aa 1-24) conjugated to KLH
Conjugate
Unconjugated
Applications
Application Notes
ELISA (solid phase: 0.1-100 μg/ml; tracer: 0.001-100 μg/ml for 30 min at RT) FC (0.5-1,0 μg/million cells in 0.1 ml) IF (0.5-1.0 μg/ml) IHC (formalin-fixed: 2-4 μg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 , for 10-20 min followed by cooling at RT for 20 minutes)
Target
Alternative Names
ACTH; Adrenocorticotropic hormone
Citations
Publication ()
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