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Fluorescence in Situ Hybridization (FISH) Services refer to a range of techniques and products that utilize fluorescently labeled DNA or RNA probes to detect and localize specific nucleic acid sequences within cells or tissues. Creative Diagnostics employs extensive knowledge of FISH to deliver services that are specifically designed to meet each customer's project needs. We are able to help you with optimizing hybridization conditions, signal specificity and clarity, as well as providing high sensitivity and reproducibility. FISH is commonly used in genetic research, clinical diagnostics, and cancer studies, among others. Our FISH services enable our clients to visualize chromosomal abnormalities, gene amplifications, or the presence of pathogens with high accuracy and reliability.
Leica TCS SP8 Confocal Microscope
Leica Bond RX Automated Research Stainer
Applied Spectral Imaging (ASI) GenASIs System
Custom Fluorescence in Situ Hybridization (FISH) Services provided by Creative Diagnostics focus on providing complete FISH solutions for the scientific community and clinical diagnostics with over 25 years of professional experience. Creative Diagnostics offers complete FISH services that include a full range of FISH technologies with accurate detection and localization of specific nucleic acid sequences in cells and tissues with the greatest sensitivity and specificity. The services are optimized for sample preparation, probe design, hybridization protocol and are subjected to the most stringent quality controls for the performance and reproducibility of FISH assays.Our FISH service has the following core advantages:
In order to meet the diverse needs of immunoassay and diagnostic applications, we provide comprehensive FISH Services, covering a variety of probe designs and hybridization technologies. Our services support downstream functional research, structural characterization, quantitative detection and diagnostic development. Relying on mature FISH technology and strict quality control, we ensure that the delivered FISH results are highly sensitive, specific and stable. Our main services include:
Design fluorescent probes with high specificity and good signal-to-noise ratio according to the customer's target sequence, and support the synthesis and labeling of DNA probes, RNA probes and multiple probes.
Includes the fixation and pretreatment of various samples such as cells, tissue sections, and cultures to ensure the integrity of the nucleic acid structure and facilitate the entry and binding of the probes.
Optimize the hybridization conditions (temperature, time, buffer, etc.) according to different samples and probe types to ensure specific binding and signal intensity.
Use a fluorescence microscope (confocal microscope, etc.) to image the hybridized samples, capture high-quality fluorescence signals, and support multi-channel multi-color fluorescence detection.
Perform quantitative and qualitative analysis on the collected images, and provide result reports such as target nucleic acid distribution, expression, and copy number changes.
Support advanced services such as multiplex fluorescence in situ hybridization, multi-color FISH, chromosome karyotype analysis, gene localization, copy number variation detection (CNV), and fusion gene detection.
Confirm sample type, target nucleic acid and project feasibility.
Design and prepare specific fluorescent probes.
Choose appropriate hybridization method to ensure efficient binding.
Optimize conditions and verify signal specificity and intensity.
Support large-scale processing and provide technical support.
This figure shows the quantitative results of FISH signal intensity of different sample groups. The results show that sample 2 has the highest signal intensity, about 400 RFU, which is significantly higher than the control group (about 100 RFU) and other sample groups. This reflects the high abundance or high expression level of its target nucleic acid sequence.
Yes, FISH technology can be used to detect low-abundance RNA or DNA targets in tissues or cells based on optimized probe design, signal amplification (such as using TSA technology) and hybridization conditions. We provide a variety of enhancement solutions to meet the needs of sensitive detection.
We support fully customized FISH probe design services, covering gene fusions, copy number variations, site mutations, etc. By referring to the sequence or chromosome location information provided by the customer, we can accurately synthesize targeted probes and perform pre-validation.
Yes, we have mature technologies for FISH detection in 3D organoids and FFPE samples, including penetration optimization, anti-background treatment and tissue clearing steps to ensure signal specificity and tissue structural integrity.
Yes. We can conduct FISH-IHC or FISH-IF combined staining experiments to achieve co-localization observation of mRNA/DNA and protein, which is particularly suitable for studies such as tumor microenvironment and stem cell characterization.
We provide multiplex FISH and automated high-throughput platform support, which can detect up to 5-10 targets simultaneously on one slide, and are widely used in cell line screening, gene expression profiling and clinical typing.
Welcome to our FISH Services! As a professional immunology service provider, Creative Diagnostics is committed to providing you with full-process FISH service support from scientific research to industrial amplification. No matter which stage of R&D you are in, our team will provide strong technical support and assistance for your scientific research innovation. Please feel free to contact us to start an efficient and reliable FISH service experience.
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