Watermelon Mosaic Virus - 2 (WMV-2) ELISA Kit (DEIAPV294)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
500T/1000T/5000T
Sample
host plants
Intended Use
The test can be used to detect WMV-2 in infected host plant tissues and seeds.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant leaf tissues diluted at 1:810 - 1:2430. This test can be used to detect WMV-2 in seeds.
General Description
Watermelon mosaic virus (WMV) also known as Marrow mosaic virus, Melon mosaic virus, and until recently Watermelon mosaic virus type 2 (WMV-2), is a plant pathogenic virus that causes viral infection (sometimes referred to as watermelon Mosaic disease) in many different plants. First described on squash in Florida, WMV arose from a unique recombination of genetic material contributed by Soybean mosaic virus (SMV) and Bean common mosaic virus (BCMV) along with Peanut Stripe virus (PSV).
Watermelon mosaic virus 2, A virus with RNA-containing flexuous filamentous particles c. 760 nm long. It induces cylindrical (pinwheel) inclusions in the cytoplasm of host cells. It is readily mechanically transmissible, is transmissible by many species of aphid in a non-persistent manner and has a moderately wide host range. It causes diseases of various cucurbits, and is also found in natural infections of several leguminous and malvaceous species. Widely distributed throughout the world. Causes mosaic and mottle diseases of cantaloupe, cucumber, pumpkin, squash and watermelon. Reduces fruit production and quality in squash and other cucurbits. Also causes mottle diseases of pea and occurs naturally in various leguminous, malvaceous, and chenopodiaceous weeds, ornamentals and crop plants.
Standard Curve

Citations


Have you cited DEIAPV294 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


Characterization of a soybean mosaic virus variant causing different diseases in Glycine max and Nicotiana benthamiana

ARCHIVES OF VIROLOGY

Authors: Jiang, Hua; Li, Kai; Dou, Daolong; Gai, Junyi

We discovered a soybean mosaic virus (SMV) variant (4278-1) that caused systemic infections in Nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. The virus had a particle morphology and incubation period similar to those of other SMV isolates but differed from them in the leaf symptoms it caused when infecting soybean and N. benthamiana. The genome of this variant consisted of a 9994-nt single-stranded RNA, which was different from most of the other known SMV isolates (approximately 9600 nt). Interestingly, we found evidence that two recombination events (nt 1-476 and nt 1145-1349) had occurred between 4278-1 and a watermelon mosaic virus analogue (WMV analogue), in the 5' untranslated region and the P1 cistron.

Identification, distribution and incidence of viruses in field-grown cucurbit crops of Iran

PHYTOPATHOLOGIA MEDITERRANEA

Authors: Bananej, Kaveh; Vahdat, Aisan

A survey of viruses in the major cucurbit-growing areas of 17 provinces in Tran was conducted in 2005 and 2006. A total of 1699 leaf samples were collected from melon, squash, cucumber and watermelon plants showing various virus-like symptoms. Screening for 11 cucurbit viruses by double-antibody sandwich ELISA (DAS-ELISA) or RT-PCR, found that 71% of the samples were infected by at least one virus, of which Cucurbit aphid-borne yellows virus (CABYV) was the most common overall, occurring in 49, 47, 40, and 33% of cucumber, squash, melon, and watermelon samples respectively. The second most common virus on melon and watermelon was Watermelon mosaic virus (WMV) (incidence 30-33%); on cucumber, Cucumber mosaic virus (CMV)(33%); and on squash, Zucchini yellow mosaic virus (ZYMV)(38%). To our knowledge, this is the first report of Melon necrotic spot virus (MNSV) and Zucchini yellow fleck virus (ZYFV) in Iran. Mixed infections Occurred in 49% of symptomatic samples. Mixed infections were relatively frequent in squash (58%) and melon (55%). The most frequent double infections were WMV+CABYV and ZYMV+CABYV in melon, squash and cucumber, followed by WMV+ZYMV. In watermelon, the most frequent double infection was WMV+ZYMV, followed by WMV+CABYV. The high frequency of CABYV, WMV and ZYMV in the samples assayed on all four cucurbit crops and in all areas surveyed, as well as the detection of Watermelon chlorotic stunt virus (WmCSV) and Cucumber vein yellowing virus (CVYV) in northern and Southern Iran, suggest that these viruses represent a potential threat to cucurbit crops in Iran.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket