White Clover Mosaic Virus (WClMV) ELISA Kit (DEIAPV301)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Intended Use
The test can be used to detect WClMV in infected host plants.
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
General Description
White clover mosaic virus (WClMV) is a plant pathogenic virus in the genus Potexvirus and the family Alphaflexiviridae. WClMV is a filamentous, flexuous rod, 480 nm in length and 13 nm wide.
The virus is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made from a single viral encoded protein. The genome has been completely sequenced and is 5845 nucleotides long. It is transmitted by mechanical inoculation, contact between plants and sometimes by seed (6% in Trifolium pretense). No insect vector is known.
Its major host is clover (Trifolium spp). It first reported in Trifolium repens in 1935. In the western United States and south western Canada it had been found in clover in a mixed infection with another potexvirus, Clover yellow mosaic virus. It is also known to infect peas (Pisum sativum), faba beans (Vicia faba), green beans (Phaseolus vulgaris), cowpea (Vigna unguiculata), cucumbers (Cucumis sativus), squash ( Cucurbita pepo ), and tomatoes (Lycopersicon esculentum).
This virus is believed to be distributed in temperate regions worldwide.
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