VZV IgM ELISA Kit

Name: VZV IgM ELISA Kit
SKU: DEIA500
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

VZV IgM ELISA Kit is intended for in vitro diagnosis of VZV associated diseases, namely varicella and herpes zoster. The diagnostic kit can also be utilized for differential diagnosis of neuroinfections, infections of eye and skin exanthematous diseases.

Contents of Kit

1. ELISA break-away strips (colourless)
2. Positive control serum
3. Negativecontrol serum
4. Calibrator
5. RF sorbent
6. Anti-human IgG antibodies
7. Wash buffer
8. Dilution buffer (DB)
9. Chromogenic substrate (TMB substrate)
10. Stop solution

Storage

1. Store the kit reagents at +2 to +10°C, in a dry place and protected from the light. Store unused strips in the sealable pouch and keep the desiccant inside.
2. Store undiluted serum samples at +2 to +10°C up to one week. For longer period make aliquots and keep them at -20°C. Avoid repeated thawing and freezing.
3. Do not store diluted samples and a diluted Px-conjugate. Always prepare fresh.
4. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately.
5. Expiration date is indicated at the ELISA kit label and at all reagent labels.

Precision

Intra assay variability: 3.1-4.2%
Inter assay variability: 2.3-13.1%

Sensitivity

Evaluation was performed by the comparing the VIDITEST kit with two other commercial ELISA tests and with indirect immunofluorescence test.

General Description

Varicella zoster virus (VZV) is one of eight herpes viruses known to infect humans (and other vertebrates). It commonly causes chicken-pox in children and Herpes zoster (shingles) in adults and rarely in children.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Reagents for VZV Assay Development and Vaccine Research

QA & Reviews

Customer Reviews

References

Relationships of varicella zoster virus (VZV)-specific cell-mediated immunity and persistence of VZV DNA in saliva and the development of postherpetic neuralgia in patients with herpes zoster

JOURNAL OF MEDICAL VIROLOGY

Authors: Park, Seong Yeon; Kim, Ji Yeun; Kwon, Ji-Soo; Jeon, Na Young; Kim, Min-Chul; Chong, Yong Pil; Lee, Sang-Oh; Choi, Sang-Ho; Kim, Yang Soo; Woo, Jun Hee; Kim, Sung-Han

Abstract

There are no surrogate markers for the development of postherpetic neuralgia (PHN) in patients with herpes zoster (HZ). All patients with HZ were prospectively enrolled to evaluate the associations of saliva varicella zoster virus (VZV) DNA persistence and VZV-specific cell-mediated immunity (CMI) with the development of PHN. Slow clearers were defined if salivary VZV DNA persisted after day 15. Salivary VZV was detected in 60 (85.7%) of a total of 70 patients with HZ on initial presentation. Of 38 patients for whom follow-up saliva samples were available, 26 (68.4%) were classified as rapid clearers and 12 (31.6%) as slow cleares. Initial VZV-specific CMI was lower in slow clearers than rapid clearers (median 45 vs 158 spot forming cells/10(6) cells, P = .02). Of the 70 patients with HZ, 22 (31.4%) eventually developed PHN. Multivariate analysis showed that slow clearers (OR, 15.7, P = .01) and lower initial VZV-specific CMI (OR, 13.8, P = .04) were independent predictors of the development of PHN, after adjustment for age and immunocompromised status. Initial low VZV CMI response and persistence of VZV DNA in saliva may be associated with the development of PHN.

Acute urinary retention and progressive paraplegia with genital infection: A case report of transverse myelitis

HONG KONG JOURNAL OF EMERGENCY MEDICINE

Authors: Kim, Eun; Lee, Duk Hee

Abstract

Introduction: Transverse myelitis (TM) is a rare inflammatory disorder involving single or multiple spinal segments. There are various Infectious and parainfectious causes. Case presentation: We report the case of 29-year-old female who presented to an emergency department with acute urinary retention, progressive headache and sensory and motor deficits with a genital infection. Cerebrospinal fluid (CSF) analysis revealed WBC 75/mu L, protein 96 (15-40 mg/dL), and VZV IgG positive. The magnetic resonance image (MRI) of the spine revealed acute transverse myelitis of C4, C6/C7 and T2-T3, T4-T7. She was treated with steroid pulse therapy, intravenous antiviral therapy, antibiotics, and rehabilitation. Urinary symptoms resolved in six days and her motor deficit resolved. She had mild numbness in the thigh area 2 months later. Discussion: Identification of the causes and diagnosis of TM are challenging because there are variable clinical signs and numerous potential pathogens. Conclusion: It is essential to diagnosis TM in the early and timely phase, careful, detailed history and thorough physical examination by emergency physicians.