Tuberculosis IgG/IgM/IgA ELISA Kit (DEIA1979)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
The Tuberculosis ELISA has been designed for measurement of specific IgG/IgM/IgA antibodies respectively against Mycobacterium tuberculosis in serum and plasma. Further applications in other body fluids are possible and can be provided on request.
Contents of Kit
1. Microtiter strips
2. Standards 1-4
3. Serum diluent
4. Enzyme conjugate
5. TMB substrate
6. Stop solution
7. Washing buffer
Storage
Reagents must bestored at 2-8°C. For more detailed information, please download the following document on our website.

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References


Evaluation of Genotoxicity of Perchlozone, Antituberculous Drug

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE

Authors: Sosedova, L. M.; Vokina, V. A.; Kapustina, E. A.; Bogomolova, E. S.

Experimental studies of Perchlozone, an antituberculous drug with manifest inhibitory activity towards Mycobacterium tuberculosis and Mycobacterium bovis, were carried out. Genotoxicity of Perchlozone was evaluated by the DNA comet method on liver and lung tissues and blood cells after 14-day inhalation exposure of rats. The level of DNA aberrations in response to inhalations of the drug in a concentration of 102.6 +/- 13.7 mg/m(3) increased in lung tissue but not in the blood cells or liver. These results indicated genotoxic activity of antituberculous drug Perchlozone.

Identification of B cell antigenome in Mycobacterium bovis by immunoproteomic analysis

ACTA VETERINARIA HUNGARICA

Authors: Cho, Yun Sang; Lee, Sang Eun; Jang, Youngboo; Jung, Sukchan; Kim, Jong Man

Bovine tuberculosis (bTB) is a common zoonosis prevalent in many countries with grave economic consequences. Most developed and developing countries have implemented the test-and-slaughter policy to protect public health and reduce economic losses in the cattle industry. The official diagnosis of bTB is based on assays dependent on cell-mediated immunity (CMI). CMI-based diagnosis demonstrates diagnostic incapability at late stages of infection, which could be overcome by diagnosis based on humoral immunity (HI). Therefore, there is an urgent need to identify and define the B cell antigenome of Mycobacterium bovis. In this study, the B cell antigenome of culture filtrate proteins (CFP) was defined by mass spectrometry-based proteomics technology. Four spots were detected on 2-dimensional gel electrophoresis (2-DE) against M. bovis-positive serum in an immunoblotting experiment. Twenty-one proteins were identified in four spots by proteomic tools, such as Mb2900, Mb2898, Mb0448, Mb3834c, Mb1918c, Mb0134c, Mb0358 and Mb1868c, which are known B cell antigens, including 13 new proteins, i.e. Mb3751, Mb2006c, Mb3276c, Mb2244, Mb1164c, Mb2553c, Mb2946c, Mb1849c, Mb1511c, Mb1034c, Mb2616c, Mb0854c and Mb2267. These new proteins identified by 2-DE and immunoblotting were the B cell antigens used in developing serological diagnostic methods based on HI to bTB.

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