Trimethoprim ELISA Kit (DEIA037)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Species Reactivity
Intended Use
The Trimethoprim ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trimethoprim in honey.
Contents of Kit
1. Microwell plate: 1 x 96 wells
2. Trimethoprim Standards: 6 vials;
3. High concentration Trimethoprim Standards: 1 x 2 mL
4. Antibody Solution: 1 x 7 mL
5. HRP Conjugate Antibody: 1 x 12 mL
6. Sample Diluent(10x): 1 x 30 mL
7. Wash Solution (20X) Concentrate: 1 x 40 mL
8. TMB Solution A: 1 x 7 mL
9. TMB Solution B: 1 x 7 mL
10. TMB Stop Solution: 1 x 7 mL
Store the kit at 2-8°C. For more detailed information, please download the following document on our website.
Detection Range
0.5-80 ppb
Detection Limit
Honey: 2 ppb
0.5 ppb


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Indirect Photodegradation of Sulfamethoxazole and Trimethoprim by Hydroxyl Radicals in Aquatic Environment: Mechanisms, Transformation Products and Eco-Toxicity Evaluation


Authors: Yang, Jiaoxue; Lv, Guochun; Zhang, Chenxi; Wang, Zehua; Sun, Xiaomin

The bacteriostatic antibiotics, sulfamethoxazole (SMX) and trimethoprim (TMP), have frequently been found in wastewater and surface water, which raises the concerns about their ecotoxicological effects. The indirect photochemical transformation has been proven to be an efficient way to degrade SMX and TMP. In this study, the reaction mechanisms of the degradation by SMX and TMF by OH radicals were investigated by theoretical calculations. Corresponding rate constants were determined and the eco-toxicity of SMX and TMP and its degradations products were predicted using theoretical models. The results indicate that the most favorable pathways for the transformation of SMX and TMP are both center dot OH-addition reaction of benzene ring site with lowest Gibbs free energy barriers (6.86 and 6.21 kcal mol(-1)). It was found that the overall reaction rate constants of center dot OH-initial reaction of SMX and TMP are 1.28 x 10(8)M(-1)s(-1)and 6.21 x 10(8)M(-1)s(-1)at 298 K, respectively. When comparing the eco-toxicity of transformation products with parent SMX and TMP, it can be concluded that the acute and chronic toxicities of the degraded products are reduced, but some products remain harmful for organisms, especially for daphnid (toxic or very toxic level). This study can give greater insight into the degradation of SMX and TMP by center dot OH through theoretical calculations in aquatic environment.

Occurrence of antibiotics and risk of antibiotic resistance evolution in selected Kenyan wastewaters, surface waters and sediments


Authors: Kairigo, Pius; Ngumba, Elijah; Sundberg, Lotta-Riina; Gachanja, Anthony; Tuhkanen, Tuula

Active pharmaceutical ingredients, especially antibiotics, are micropollutants whose continuous flow into hydrological cycles has the potential to mediate antibiotic resistance in the environment and cause toxicity to sensitive organisms. Here, we investigated the levels of selected antibiotics in four wastewater treatment plants and the receiving water bodies. The measured environmental concentrations were compared with the proposed compound-specific predicted no-effect concentration for resistance selection values. The concentration of doxycycline, amoxicillin, sulfamethoxazole, trimethoprim, ciprofloxacin and norfloxacin within the influents, effluents, surface waters and river sediments ranged between 0.2 and 49.3 mu gL(-1), 0.1 to 21.4 mu gL(-1); (<)0.1 and 56.6 mu gL(-1); and 1.8 and 47.4 mu gkg(-1), respectively. Compared to the effluent concentrations, the surface waters upstream and downstream one of the four studied treatment plants showed two to five times higher concentrations of ciprofloxacin, norfloxacin and sulfamethoxazole. The risk quotient for bacterial resistance selection in effluent and surface water ranged between (<)0.1 and 53, indicating a medium to high risk of antibiotic resistance developing within the study areas. Therefore, risk mitigation and prevention strategies are a matter of priority in the affected areas. (C) 2020 Elsevier B.V. All rights reserved.

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