Toxocara ELISA Kit (DEIA05740)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
For the qualitative screening of serum IgG antibodies to Toxocara using an Enzyme-Linked Immunosorbent Assay (ELISA) technique.
Contents of Kit
1. Test Strips-Microwells containing Leptospira antigen-96 test wells in a test strip holder.
2. Enzyme Conjugate-One bottle containing 11 mL of anti-human IgG antibody conjugated to peroxidase.
3. Positive Control-One vial containing 2 mL of diluted surrogate positive control.
4. Negative Control-One vial containing 2 mL of diluted negative human serum.
5. Chromogen-One bottle containing 11 mL of the chromogen tetramethylbenzidine .
6. Wash Concentrate -One bottle containing 25 mL of concentrated buffer and surfactant.
7. Dilution Buffer-Two bottles containing 30 mL of buffered protein solution.
8. Stop Solution-One bottle containing 11 mL of 0.73 M phosphoric acid.
Storage
Reagents, strips and bottled components:
Store at 2-8°C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature. For more detailed information, please download the following document on our website.
Sensitivity
Sensitivity of 87.5% (21/24)

Citations


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References


First record of Pseudoterranova decipiens (Nematoda, Anisakidae) infecting the Red spot emperor Lethrinus lentjan in the Red Sea

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA

Authors: Al Quraishy, Saleh; Abdel-Gaber, Rewaida; Dkhil, Mohamed Abdel Monem

The current parasitological study was carried out to investigate helminth parasites infecting the Red spot emperor Lethrinus lentjan inhabiting Hurghada City at the Gulf of Suez, Red Sea, Egypt. Third-stage larvae of nematode parasite was isolated from the intestine as well as body cavity of the examined fish. Light and scanning electron microscopy revealed that this parasite belonged to Anisakidae family within the genus Pseudoterranova. The present species is named Pseudoterranova decipiens based on the presence of triangular mouth aperture with prominent boring teeth and soft swellings of the cuticle, long muscular esophagus, ventrally excretory pore, and narrow transverse slit of anal opening followed by a short mucron. The morphological characteristics of this species were confirmed by molecular analysis of 18S rDNA gene region of the present parasite. It demonstrated a close identity >= 89% with taxa under family Anisakidae, 85% with Raphidascarididae, and 79-84% with Toxocaridae. A preliminary genetic comparison between gene sequence of the present parasite and other oxyurid species placced it as a putative sister taxon to other Pseudoterranova decipiens described previously. ibis study demonstrated that the 18S rDNA gene region of Pseudoterranova decipiens yielded a unique sequence that confirmed its taxonomic position in Anisakidae.

The complete mitochondrial genome of Toxascaris leonina: Comparison with other closely related species and phylogenetic implications

INFECTION GENETICS AND EVOLUTION

Authors: Liu, Guo-Hua; Zhou, Dong-Hui; Zhao, Lei; Xiong, Rong-Chuan; Liang, Jian-Ying; Zhu, Xing-Quan

Adults of Toxascaris leonina (Nematoda: Ascarididae) live in the gastrointestinal tract of both dogs and cats, and cause significant economic losses and potential public health problem worldwide. Although many studies have given insights into this significant pathogen, to date, the complete mitochondrial (mt) genome sequence is still not available for T. leonina. Here, we sequenced the complete mt genome of T. leonina. This AT-rich (71.53%) mt genome (14,310 bp) is circular and consists of 36 genes, including 12 genes for proteins, 2 genes for rRNA and 22 genes for tRNA. All mt genes of T. leonina are transcribed in the same direction. The gene order is the same as those of Ascaris spp. (Ascarididae), Toxocara spp. (Toxocaridae), Anisakis simplex and Contracaecum rudolphii B (Anisakidae), but distinct from that of Ascaridia spp. (Ascaridiidae). Phylogenetic analyses using concatenated amino acid sequences of 12 protein-coding genes by Bayesian inference (BI) showed distinct groups with high statistical support, and our data confirm that T. leonina is a member of the Ascarididae, and that this family is more closely related to the Toxocaridae rather than the Anisakidae within the Ascaridoidea. The determination of mt genome sequences of T. leonina provides novel genetic markers for studies into the systematics, population genetics and epidemiology of this parasite. (C) 2013 Elsevier B. V. All rights reserved.

Huy, V. Q., et al. "Development and Testing of Trial Samples for Specific Serodiagnosis of Fasciola gigantica via External Quality Assessment." J Bacteriol Parasitol 10.351 (2019): 2.

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