Toscana Virus Nucleocapsid (DAG-P2281)

Toscana Virus Nucleocapsid (aa 1 - 180), recombinant protein from E. coli

Product Overview
Toscana Virus Nucleocapsid full length protein
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
34 kDa including tags
Cellular Localization
Virion. Note: Internal protein of virus particle.
Procedure
None
Purity
>95% by SDS-PAGE .
Format
Liquid
Buffer
pH: 8.00Constituents: 45% Phosphate Buffer, 48.5% Urea, 5.8% Sodium chloride, 0.1% C13E10
Preservative
None
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. pH: 8.00Constituents: 45% Phosphate Buffer, 48.5% Urea, 5.8% Sodium chloride, 0.1% C13E10
Introduction
Toscana virus (TOSV) is an arbovirus (arthropod-borne virus) belonging to Bunyaviridae, a family of negative-stranded, enveloped RNA viruses. The virus can be transmitted to humans by the bite of an infected sandfly of the genus Phlebotomus. Toscana is no
Antigen Description
At least five proteins have been identified in Toscana virus-infected cells: nucleoprotein N, glycoproteins G1 and G2, a large protein (L) assumed to be a component of the polymerase, and two nonstructural proteins, NSm and NSs.
Keywords
N; Nucleocapsid protein; Protein N; TOSV Nucleocapsid

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References


Immunoblot detection of antibodies to Toscana virus

JOURNAL OF MEDICAL VIROLOGY

Authors: Schwarz, TF; Gilch, S; Pauli, C; Jager, G

Sera from patients with sandfly fever caused by Toscana virus (TOSV) infection were tested by immunoblot for specific antibody response to TOSV derived from infected Vero-E6 cells. The 28 kDa TOSV nucleoprotein (N) was identified as the major immunodominant protein recognized by immunoblot. In sera of patients with acute TOSV infection, specific antibodies of the IgM, IgA, and IgG class were detected. Using sandfly fever virus, serotypes Sicilian (SFSV) and Naples (SFNV), as antigens for immunoblot, TOSV antibody-positive sera cross-reacted with the corresponding N proteins. These sera reacted for IgM and IgG by SFSV immunoblot, and for IgM by SFNV immunoblot. The diagnosis of sandfly fever may be confirmed by TOSV immunoblot. (C) 1996 Wiley-Liss, Inc.

Canine Infections and Partial S Segment Sequence Analysis of Toscana Virus in Turkey

VECTOR-BORNE AND ZOONOTIC DISEASES

Authors: Dincer, Ender; Karapinar, Zeynep; Oktem, Mert; Ozbaba, Merve; Ozkul, Aykut; Ergunay, Koray

Introduction: Toscana virus (TOSV) is a sandfly-borne bunyavirus with a significant public health impact. Preliminary studies have revealed TOSV exposure in dogs and they were suggested as potential reservoirs. This study was performed to characterize canine TOSV infections in an endemic region. Sequencing of TOSV small (S) segment in several previously identified specimens was also undertaken to reveal viral genealogy. Materials and Methods: Canine and feline plasma were collected in several districts of Mersin province, Mediterranean Anatolia, Turkey, during May-September, 2015. Phlebovirus RNA was screened through two nested polymerase chain reaction (PCR) assays, targeting S and large (L) segments of the viral genome. A kinetoplast minicircle nested PCR was employed for Leishmania DNA detection and typing. Previously collected TOSV-positive specimens from humans, dogs, cats, and sandflies from various regions in Turkey and Cyprus were further evaluated through the S segment PCR. All amplicons were characterized through sequencing. Results: A total of 210 specimens that comprise canine (76.2%) and feline (23.8%) plasma were screened. In three (1.9%) and two (1.3%) canine specimens, TOSV and Leishmania nucleic acids were detected, respectively. The TOSV strains were characterized as genotype B, and Leishmania infantum was identified in positive specimens. Twenty-four partial S segment sequences were amplified, which demonstrated a maximum intramural diversity of 3.88% in the nucleotide level. Sequence comparisons revealed significant similarities to particular genotype B strains characterized in Spain and France, whereas a notable divergence was observed among several TOSV strains. Single or recurrent amino acid substitutions were noted in eight residues of the viral nucleocapsid. Discussion: Canine infections of TOSV genotype B, with temporal and spatial association with L. infantum, were detected. Divergent TOSV S segment sequences with amino acid substitutions, presumably associated with host adaptation, were observed.

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