Florfenicol & Thiamphenicol ELISA Kit

A method for quantitative detection of florfenicol by colloidal gold lateral flow immunoassay was developed. The experimental conditions including pH value, concentrations of antibody in the process of conjugation between the colloidal gold and antibody, amount of gold. labeled antibody, concentration of the antigen sprayed on test lines (T line), and detection time were optimized. With a colloidal gold strip reader, the signal intensity of T lines and control lines (C line) on lateral flow strips was recorded. The T/C ratio of negative control and positive samples was defined as B-0 and B-x, and the standard curve was established by plotting the B-x/B-0 ratio against the concentration of florfenicol. This assay showed a good linear range from 0. 1 to 1. 5 ng/mL with the limit of detection of 0. 08 ng/mL, while the result could be obtained within 15 min. The result showed that this quantitative method was convenient and rapid, and could be used in screening a large amount of samples on site.

A rapid and sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method to quantify thiampllenicol (TAP), floifenicol (FF), and floifenicol amine (FFA) in swine muscle is described An immunoaffinity chromatography (IAC) column based oil polyclonal antibodies and protein A-sepharose CL 4B was used to clean-up extracted samples IAC optimized conditions were found that allowed the IAC to be reused for selective binding of TAP, FF, and FFA The dynamic column capacity was mote than 512 ng/mL of gel after being used for 15 cycles From fortified swine muscle samples at levels of 04-50 ng/g, the average recoveries were 85 2-989% with infra- and inter-clay variations less than 9 8% and 12 4%, respectively The limit of quantitation ranged from 0 4 to 4 0 mu g/kg (C) 2009 Elsevier B.V. All rights reserved