The test can be used to detect TYMV in infected host plants.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
General Description
Turnip yellow mosaic virus (TYMV) is an isometric tymovirus of the family Tymoviridae. Its host range is confined almost entirely to plants in the Brassica genus in western Europe, which includes cabbages, cauliflower and broccoli. Infection causes bright yellow mosaic disease showing vein clearing and molting of plant tissues. It is transmitted by sap as well as a host of insect vectors. The most prominent of these are in the Phyllotreta and Psylliodes genera of flea beetles, although Phaedon cochleariae and its larva have also been known to help spread this virus. The larva lose their ability to transmit the disease once they reach the pupal stage, suggesting a mechanical infection process.
Standard Curve
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