Tetrodotoxin ELISA Test Kit (DEIANJ48)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Water; Fish
Intended Use
The Tetrodotoxin indirect competitive ELISA is an immunoassay for the quantitative and sensitive detection of Tetrodotoxin in water samples and puffer fish samples .
Storage
The Tetrodotoxin ELISA kit in its original packaging can be used until the end of the month indicated on the box label when stored at 2- 8°C.
Sensitivity
10.0 ng/mL
General Description
Tetrodotoxin (TTX) is a powerful neurotoxin, which is tolerance to heat, salt and cooking .Minimum lethal dose for human is about 0.5mg/60 kg of body weight, The toxicity is 1000 times great than the sodium cyanide. Every year many people are ill due to improper eating or eating puffer fish. Therefore, it is significance to accurate detection of tetrodotoxin in puffer fish in order to prevention and control of tetrodotoxin poisoning . The detection method is sensitive, fast, simple and specific, only need a small amount of fish sampling.

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References


Alfaxalone Causes Reduction of Glycinergic IPSCs, but Not Glutamatergic EPSCs, and Activates a Depolarizing Current in Rat Hypoglossal Motor Neurons

FRONTIERS IN CELLULAR NEUROSCIENCE

Authors: Lau, Cora; Thakre, Prajwal P.; Bellingham, Mark C.

We investigated effects of the neuroactive steroid anesthetic alfaxalone on intrinsic excitability, and on inhibitory and excitatory synaptic transmission to hypoglossal motor neurons (HMNs). Whole cell recordings were made from HMNs in brainstem slices from 7 to 14-day-old Wistar rats. Spontaneous, miniature, and evoked inhibitory post-synaptic currents (IPSCs), and spontaneous and evoked excitatory PSCs (EPSCs) were recorded at -60 mV. Alfaxalone did not alter spontaneous glycinergic IPSC peak amplitude, rise-time or half-width up to 10 mu M, but reduced IPSC frequency from 3 mu M. Evoked IPSC amplitude was reduced from 30 nM. Evoked IPSC rise-time was prolonged and evoked IPSC decay time was increased only by 10 mu M alfaxalone. Alfaxalone also decreased evoked IPSC paired pulse ratio (PPR). Spontaneous glutamatergic EPSC amplitude and frequency were not altered by alfaxalone, and evoked EPSC amplitude and PPR was also unchanged. Alfaxalone did not alter HMN repetitive firing or action potential amplitude. Baseline holding current at -60 mV with a CsCl-based pipette solution was increased in an inward direction; this effect was not seen when tetrodotoxin (TTX) was present. These results suggest that alfaxalone modulates glycine receptors (GlyRs), causing a delayed and prolonged channel opening, as well as causing presynaptic reduction of glycine release, and activates a membrane current, which remains to be identified. Alfaxalone selectively reduces glycinergic inhibitory transmission to rat HMNs via a combination of pre- and post-synaptic mechanisms. The net effect of these responses to alfaxalone is to increase HMN excitability and may therefore underlie neuro-motor excitation during neurosteroid anesthesia.

Tetrodotoxin-Sensitive Sodium Channels Mediate Action Potential Firing and Excitability in Menthol-Sensitive Vglut3-Lineage Sensory Neurons

JOURNAL OF NEUROSCIENCE

Authors: Griffith, Theanne N.; Docter, Trevor A.; Lumpkin, Ellen A.

Small-diameter vesicular glutamate transporter 3-lineage (Vglut3(lineage)) dorsal root ganglion (DRG) neurons play an important role in mechanosensation and thermal hypersensitivity; however, little is known about their intrinsic electrical properties. We therefore set out to investigate mechanisms of excitability within this population. Calcium microfluorimetry analysis of male and female mouse DRG neurons demonstrated that the cooling compound menthol selectively activates a subset of Vglut3(lineage) neurons. Whole-cell recordings showed that small-diameter Vglut3(lineage) DRG neurons fire menthol-evoked action potentials and exhibited robust, transient receptor potential melastatin 8 (TRPM8)-dependent discharges at room temperature. This heightened excitability was confirmed by current-clamp and action potential phase-plot analyses, which showed menthol-sensitive Vglut3(lineage) neurons to have more depolarized membrane potentials, lower firing thresholds, and higher evoked firing frequencies compared with menthol-insensitive Vglut3(lineage) neurons. A biophysical analysis revealed voltage-gated sodium channel (Na-v) currents in menthol-sensitive Vglut3(lineage) neurons were resistant to entry into slow inactivation compared with menthol-insensitive neurons. Multiplex in situ hybridization showed similar distributions of tetrodotoxin (TTX)-sensitive Na-v transcripts between TRPM8-positive and -negative Vglut3(lineage) neurons; however, Na-v 1.8 transcripts, which encode TTX-resistant channels, were more prevalent in TRPM8-negative neurons. Conversely, pharmacological analyses identified distinct functional contributions of Nay subunits, with Na-v 1.1 driving firing in menthol-sensitive neurons, whereas other small-diameter Vglut3(lineage) neurons rely primarily on TTX-resistant Nay channels. Additionally, when Na-v 1.1 channels were blocked, the remaining Nay current readily entered into slow inactivation in menthol-sensitive Vglut3(lineage) neurons. Thus, these data demonstrate that TTX-sensitive Na(v)s drive action potential firing in menthol-sensitive sensory neurons and contribute to their heightened excitability.

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