The test can be used to detect TRSV in infected host plans.
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant leaf tissues diluted at 1:270 - 1:810.
Tobacco ringspot virus (TRSV) is a plant pathogenic virus in the plant virus family Secoviridae. It is the type species of the genus Nepovirus. Nepoviruses are transmitted between plants by nematodes, varroa mites and honeybees. TRSV is also easily transmitted by sap inoculation and transmission in seeds has been reported. In recent cases it has also been shown to appear in bees.
Symptoms and virus inclusions of Tobacco ringspot nepovirus in the host Zamia furfuracea, the Cardboard Cycad. A. The first symptoms seen were chlorotic ringspots. With time they become necrotic. B. There are two types of inclusions found in leaf strips stained with Azure A (nucleic acid stain), one is vacuolate (Vac Inc) and the other more crystalloid (Cyst Inc - darker spots). C. Vacuolate inclusions only.