Anti-TRIAD1 polyclonal antibody (CABT-BL6330)


Host Species
Antibody Isotype
Species Reactivity
human TRIAD1 (residues 1-493)


Application Notes
WB: 0.1-0.5 µg/ml
IP: Use 2.0 µg/mg of cell extract
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.


Alternative Names
ARIH2; ariadne homolog 2 (Drosophila); ariadne (Drosophila) homolog 2; E3 ubiquitin-protein ligase ARIH2; all trans retinoic acid inducible RING finger; TRIAD1
Entrez Gene ID
UniProt ID


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Arih2 gene influences immune response and tissue development in chicken


Authors: Wu, Guanxian; Xu, Sifan; Zhang, Wanting; Liu, Yang; Wang, Qiuyuan; Man, Chaolai

Ariadne homolog 2 (ARIH2), as an E3 ubiquitin ligase, is one of the important factors involved in regulating biological functions, such as inflammation and skeletal muscle degeneration. In the present study, the full-length coding sequence of Arih2 gene was cloned from Hy-Line Brown chicken. The tissue transcriptional profiles of Arih2 gene at different developmental stages were detected using quantitative real-time PCR (qRT-PCR), and the Arih2 functional characteristics in immune response were analyzed. The results showed that the full-length coding sequence of Arih2 gene was 1473 bp, encoding 490 amino acids, and conservative between different species. The Arih2 gene was transcribed in various tissues at different developmental stages, and its transcriptional activities varied significantly between multiple tissues. With the development of chicken, Arih2 gene was basically up-regulated in heart, liver, kidney, skeletal muscle and glandular stomach, but fluctuated significantly in large intestine. In immune response, the transcriptional activities of Arih2 gene exhibited significant changes in the bursa, thymus and blood (P<0.05). The results showed that Arih2 might be a multifunctional gene involved in tissue development and immune response in chicken, and have a potential possible application as diagnostic marker for identifying immune response.

cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro


Authors: Jin, K; Mao, XO; Eshoo, MW; del Rio, G; Rao, R; Chen, D; Simon, RP; Greenberg, DA

We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.

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