TNNI2 (Monkey) ELISA Kit

Sample

EDTA Plasma, serum

Species Reactivity

Monkey

Detection Method

sELISA

Intended Use

TNNI2 (Monkey) ELISA Kit is for the quantitative measurement of TNNI2 in serum or plasma (EDTA).

Contents of Kit

1. Anti-SkM-TnI coated wells: 96 wells (provided as 12 detachable strips of 8)
2. SkM-TnI Stock: 3 vials. Lyophilized SkM-TnI (reconstitute with 0.10 ml H₂O).
Note: International import/export restrictions apply to monkey derived products. In order to avoid such restrictions the SkM-TnI standard supplied with this kit is of non monkey origin. The standard curve obtained with this material is identical to that obtained with monkey SkM-TnI.
3. Standard Diluent: 50 ml
4. Sample Diluent: 25 ml
5. 20× Wash Solution: 50 ml
6. Anti SkM-TnI HRP Conjugate: 11 ml
7. TMB Reagent: 11 ml
8. Stop Solution (1N HCl) 11 ml

Storage

Store the SkM-TnI Stock vials at or below -20°C. Store the remainder of the kit at 2-8°C. Keep the microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.

General Description

Troponin is the contractile regulating protein complex of striated muscle. It consists of three distinct polypeptides: troponin-I, troponin-C, and troponin-T. The troponin-I subunit exists in three distinct isoforms; one each in fast-twitch and slow-twitch skeletal muscle fibers, and one in cardiac muscle. Following muscle injury, troponin-I is released into the blood and measurement of troponin-I in serum or plasma provides a measurement of the extent of muscle injury. This ELISA kit uses a detection antibody that is specific for the fast twitch isoform of troponin-I, thereby allowing specific evaluation of skeletal muscle injury.

Standard Curve

Results of a typical standard curve with A450 plotted on the Y axis against cTnI concentrations on the X axis are shown below. Note: This standard curve is for the purpose of illustration only.