The test can be used to detect TNDV in infected host plants.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
General Description
Tobacco Necrotic Dwarf Virus is a virus with RNA-containing isometric particles c. 25 nm in diameter occurring in phloem cells. Transmitted by aphids in the persistent manner, but not by inoculation with sap. It has a narrow host range. Recorded only from Japan. Causes stunting, and premature yellowing and death of the lower and intermediate-aged leaves, of tobacco (Nicotiana tabacum) plants . Causes faint vein yellowing in spinach.
Standard Curve
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