TLR2 (Human) ELISA Kit (DEIA4318)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
biological fluids, cell culture supernatant, tissues homogenate
Species Reactivity
Human
Intended Use
This immunoassay kit allows for the in vitro quantitative determination of human TLR2 concentrations in serum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids.
Contents of Kit
1. Assay plate
2. Standard
3. Sample Diluent
4. Assay Diluent A
5. Assay Diluent B
6. Detection Reagent A
7. Detection Reagent B
8. Wash Buffer (25X)
9. Substrate
10. Stop Solution
Storage
The Assay Plate, Standard, Detection Reagent A and Detection Regent B should be stored at -20°C upon being received. After receiving the kit, Substrate should be always stored at 4°C. Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Detection Range
0.156 ng/mL-10 ng/mL

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References


The use of Hericium erinaceus and Trametes versicolor extracts in supportive treatment in oncology

ACTA PHARMACEUTICA

Authors: Winder, Mateusz; Bulska-Bedkowska, Weronika; Chudek, Jerzy

Substances available in nature with potential therapeutic effects are the subject of research that raises tremendous hopes for new challenges in medicine. Fungi are the most common organisms in the ecosystem and the most interesting in this respect. This review discusses two species of edible fungi, used for centuries in Eastern natural medicine, with the best-documented effect - Hericium erinaceus (He) and Trametes versicolor (Tv). The results of in vivo and in vitro studies conducted on mice and human cell lines demonstrate immunomodulatory, potentially, anticancer, anti-inflammatory and neuroregenerative effects of substances isolated from these fungi. The substances contained in the extracts of He and Tv seem to have immunomodulatory effects that may support chemotherapy. The use of these extracts is justified stronger than the other supportive treatments based on supplements.

TLR2/4 promotes PGE(2) production to increase tissue damage in Escherichia coli-infected bovine endometrial explants via MyD88/p38 MAPK pathway

THERIOGENOLOGY

Authors: Li, Tingting; Hai, Lili; Liu, Bo; Mao, Wei; Liu, Kun; Shen, Yuan; Li, Qianru; Guo, Yuli; Jia, Yan; Bao, Haixia; Cao, Jinshan

Prostaglandin E2 (PGE(2)), a lipid mediator, is released by several cell types including endometrial cells and plays a central role in bacterial infection of the endometrium during inflammation. PGE(2) production accumulated in Escherichia coli (E. coli)-infected bovine endometrial tissue, which increased E. coli-infected endometrial tissue damage. However, the mechanisms of PGE(2) accumulation in the E. coli-infected endometrium during inflammation-associated endometrial tissue damage remain unclear. This study was conducted to investigate the role of Toll-like receptors (TLRs) 2 and 4 in increased PGE(2) production in E. coli-infected endometrial tissue. E. coli and TLR2/4 agonists significantly induced cyclooxygenase-2 and microsomal prostaglandin E synthase-1 expression and PGE(2) synthesis detected by RT-PCR, Western blot, and ELISA in the endometrial tissue. The expression and synthesis were dramatically decreased by TLR4, myeloid differentiation factor88 (MyD88), and p38 mitogen-activated protein kinase (MAPK) inhibitors in E. coli-infected endometrial tissue. These inhibitors also significantly decreased proinflammatory factor (interleukin-6 and tumor necrosis factor-alpha) and damage-associated molecular pattern (high mobility group box-1 and hyaluronan-binding protein-1) release and tissue damage measured by double-label immunofluorescence in E. coli-infected endometrial explants. Our work provides in vitro evidence that TLR2/4-MyD88/p38 MAPK promotes PGE(2) synthesis and E. coli-infected endometrial tissue damage, which may be useful for improving PGE(2)-based therapies for endometritis. (C) 2020 Elsevier Inc. All rights reserved.

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