Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The TLK1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
TLK1; tousled-like kinase 1; serine/threonine-protein kinase tousled-like 1; KIAA0137; PKU BETA; SNARE protein kinase SNAK; serine threonine protein kinase; PKU-beta;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Sharp, JA; Rizki, G; et al. Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae. GENETICS 171:885-899(2005).
Kelly, R; Davey, SK; et al. Tousled-Like Kinase-Dependent Phosphorylation of Rad9 Plays a Role in Cell Cycle Progression and G2/M Checkpoint Exit. PLOS ONE 8:-(2013).
COA
Certificate of Analysis
