Sulfaquinoxaline, SQX ELISA Kit (DEIA035)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
tissues, honey, serum, urine, milk
Species Reactivity
N/A
Intended Use
The Sulfaquinoxaline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfaquinoxaline in tissue, honey, serum, urine, milk.
Contents of Kit
1. Microwell plate: 1 x 96 wells
2. Sulfaquinoxaline Standards: 6 vials;
3. High concentration Sulfaquinoxaline Standards: 1 x 2 mL
4. Antibody Solution: 1 x 7 mL
5. HRP Conjugate Antibody: 1 x 12 mL
6. Sample Diluent(10x): 1 x 30 mL
7. Wash Solution (20X) Concentrate: 1 x 40 mL
8. TMB Solution A: 1 x 7 mL
9. TMB Solution B: 1 x 7 mL
10. TMB Stop Solution: 1 x 7 mL
Storage
Store the kit at 2-8°C. For more detailed information, please download the following document on our website.
Detection Range
1-81 ppb
Detection Limit
Tissue: 2 ppb
serum, urine: 4 ppb
Milk: 20 ppb
Honey: 1 ppb
Sensitivity
1 ppb

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References


Development and validation of an HPLC confirmatory method for the residue analysis of four sulphonamides in cow's milk according to the European union decision 2002/657/EC

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

Authors: Samanidou, Victoria F.; Tolika, Evanthia P.; Papadoyannis, Ioannis N.

In the present study, an HPLC method was developed and validated for the determination of four sulphonamides: sulphadiazine (SDZ), sulphaquinoxaline (SQX), suplhamethazine (SMTH), and sulphadimethoxine (SDM). Two of them, SQX and SDM, were determined in cow's milk. The analytical column, a Kromasil, C(18) 5 mu m, 250 x 4 mm analytical column, was operated at ambient temperature. The mobile phase, a mixture of 0.5% acetic acid as solvent A, CH(3)CN as solvent B, and CH(3)OH was delivered to the analytical column according to a gradient program. PDA detection was performed for the detection and confirmation of separated analytes with monitoring at 260nm. Method validation was performed by means of intra-day (n=5) and inter-day accuracy and precision (n=5), sensitivity, and linearity. Limits of detection (LOD) and limits of quantification (LOQ) were 13 and 40 mu g/kg, respectively. Solid phase extraction was applied to remove all matrix interference from milk samples after deproteinization with 8M HCl. High extraction recoveries (>84%) were achieved using DSC-18 cartridges with CH(3)OH-0.5% CH(3)COOH as eluent. CC alpha values were 111.8 and 117.1 mu g/kg for SDM and SQX, respectively, and CC values were 116.6 and 134.0 mu g/kg, respectively. The method was applied to the analysis of twenty two milk samples from the local market. SQX was identified in seven of these samples.

Immunogenicity of novel sulfadimethoxide conjugates

AFRICAN JOURNAL OF BIOTECHNOLOGY

Authors: Chen, Lei; Su, Jianyu; Zhang, Xiangzhai; Li, Lin; He, Xiaowei

Sulfadimethoxine (SDM) is an antibiotic commonly added to animal feeds. Anti-SDM antibodies are useful for the detection of residual SDM in foods, feeds and biological fluids by ELISA. In this study, we show that SDM is immunogenic in rabbits when it is conjugated with soy 11S globulin or with beta-amylase. Rabbit anti-SDM antibodies obtained by immunization with SDM-11S and SDM-beta-amylase displayed low cross-reactivity against other sulfonamides, including sulfamethazine (SM2), sulfasulfonamides (SN), sulfadiazine (SD), sulfamethoxypyrazine (SMP), sulfalene (SMZ), sulfaquinoxaline (SQX). Thus, soy 11S globulin and beta-amylase are suitable carriers for the induction of anti-SDM antibodies and have the advantage of being cheaper that BSA.

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