Streptavidin [FITC]

Product Overview

Streptavidin, FITC-conjugated

Target

Streptavidin

Specificity

Ficoll prepared human peripheral blood lymphocytes, CEM, HPB-MLT, Nalm-6, Jurkat, U-937, Molt-4, Raji, Daudi and KG-1 (cells expressing Fc receptors were pre-blocked with human IgG).

Nature

Synthetic

Tag/Conjugate

FITC

Recommended Usage

Streptavidin can be used in conjunction with biotinylated antibodies as an avidin/biotin labeling system for flow cytometry.

Purity

Purified streptavidin was covalently conjugated to FITC and the conjugate isolated by size exclusion chromatography. with a FITC to protein molar ratio of 9.0.

Format

Liquid

Concentration

100 μg/mL

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150 mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Preservative

0.04% Sodium Azide

Storage

Store at 2 - 5°C. Do not freeze! Protect from light. Product should retain activity for at least 12 months after shipping date when stored as recommended.

Introduction

Streptavidin is a 52.8 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7 or vitamin H). With a dissociation constant (Kd) on the order of ≈10?14 mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bionanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.

Keywords

Streptavidin