Human Soluble EphA1 ELISA Kit (DEIA-XY2210)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, EDTA plasma, cell culture supernates
Species Reactivity
Human
Intended Use
This Human soluble Ephrin Type-A Receptor 1 (EphA1) ELISA Kit contains the necessary components required for the quantitative measurement of recombinant and/or natural soluble human EphA1 from cell culture supernates, serum and plasma in a sandwich ELISA format.
Contents of Kit
1. EphA1 Microplate: 1 plate
2. EphB6 Standard: 1 vial
3. Detection Antibody-HRP Conjugate: 1 vial
4. Positive Control: 1 vial
5. Dilution Buffer: 1 bottle
6. Wash Buffer: 1 bottle
7. Substrate Solution: 1 bottle
8. Stop Solution: 1 bottle
9. Plate Sealer: 1
10. Plastic Pouch: 1
Storage
2-8°C
Precision
Intra-assay Precision: 4-6%; Inter-assay Precision: 8-10%
Detection Range
39-2500 pg/mL
Sensitivity
12 pg/mL
Standard Curve

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References


Increased DNA methylation near TREM2 is consistently seen in the superior temporal gyrus in Alzheimer's disease brain

NEUROBIOLOGY OF AGING

Authors: Smith, Adam R.; Smith, Rebecca G.; Condliffe, Daniel; Hannon, Eilis; Schalkwyk, Leonard; Mill, Jonathan; Lunnon, Katie

Although mutations within the TREM2 gene have been robustly associated with Alzheimer's disease, it is not known whether alterations in the regulation of this gene are also involved in pathogenesis. Here, we present data demonstrating increased DNA methylation in the superior temporal gyrus in Alzheimer's disease brain at a CpG site located 289 bp upstream of the transcription start site of the TREM2 gene in 3 independent study cohorts using 2 different technologies (Illumina Infinium 450K methylation beadchip and pyrosequencing). A meta-analysis across all 3 cohorts reveals consistent AD-associated hyper-methylation (p = 3.47E-08). This study highlights that extending genetic studies of TREM2 in AD to investigate epigenetic changes may nominate additional mechanisms by which disruption to this gene increases risk. (C) 2016 Elsevier Inc. All rights reserved.

The C677T polymorphism of the methylenetetrahydrofolate reductase gene and susceptibility to late-onset Alzheimer's disease

OPEN MEDICINE

Authors: Yi, Jian; Xiao, Lan; Zhou, Sheng-Qiang; Zhang, Wen-Jiang; Liu, Bai-Yan

Folate metabolism makes a crucial contribution towards late-onset Alzheimer's disease (LOAD). Moreover, methylenetetrahydrofolate reductase (MTHFR) constitutes the primary enzyme of the folate pathway. We hypothesize that there is an association of C677T polymorphism in the MTHFR gene with the susceptibility to LOAD. Previous published research has investigated the link between the MTHFR C677T polymorphisms and LOAD susceptibility; nevertheless, the findings have continued to be not only controversial, but also indecisive. Accordingly, we carried out the present meta-analysis for the assessment of the potential link that exists between the MTHFR C677T polymorphism and the susceptibility to LOAD. Furthermore, we carried out a literature search of the PubMed, EMBASE, Cochrane Library, and WanFang database up to August 10, 2018. The odds ratios (ORs) with the respective 95% confidence interval (95%CI) were put to use for the evaluation of the robustness of the link of the MTHFR C677T polymorphism with the vulnerability to LOAD. All statistical analyses were carried out using STATA 15.0. An aggregate of 14 case-control research works was retrieved, involving 2,467 LOAD patients as well as 2,877 controls. We found that a substantial link exists between C677T polymorphism and LOAD risk in a codominant framework (TC vs. CC: OR=1.22, 95%CI=1.00-1.49, P=0.049). In addition to the stratified analysis based on ethnicity, which suggested that C677T polymorphism was likely linked only to an augmented threat of LOAD in Asians, it did not exist among Caucasians. Furthermore, in the subgroup analysis carried out using APOE epsilon 4 status, a substantial increase in the susceptibility to LOAD was detected in APOE epsilon 4 carriers as well as non-APOE epsilon 4 carriers. In sum, the current meta-analysis revealed that MTHFR C677T polymorphism was associated with susceptibility to LOAD. Further extensive case-control studies are required.

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