Human Scrub Typhus IgG ELISA kit (DEIABL76)

Regulatory status: For research use only, not for use in diagnostic procedures.

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serum, plasma
Species Reactivity
Intended Use
The Scrub Typhus IgG ELISA test for exposure to Orientia tsutsugamushi (OT; formerly Rickettsia) is an ELISA assay system for the detection of IgG antibodies in human plasma/serum to OT derived recombinant antigen (1-10). This test is to aid in the diagnosis of human exposure to OT species. It is not intended to screen blood or blood components, and is for research use only. Not for use in diagnostic procedures.
Contents of Kit
1. Scrub Typhus ELISA Plate
2. Sample Dilution Buffer for Scrub Typhus
3. Scrub Typhus IgM Positive Control
4. Scrub Typhus Negative Control
5. Ready to Use Enzyme Conjugate-HRP for Scrub Typhus IgM
6. 10X Wash Buffer
7. Wash Solution
8. Liquid TMB Substrate
9. Stop Solution
Stable at 2-8°C until the expiration date.
Performance Characteristics
Serum and Plasma Comparisons: The assay described here has been optimized with serum. Care should be taken on the quality of sample. Particulate, lipemic, and aged samples should not be used. Use of freshly drawn sample is preferred.
Specificity and sensitivity: Detail specificity and sensitivity have been not been established. Limited studies have been performed.

The results on the table below must be obtained using provided positive and negative control to calculate discrimination capacity of the assay: Non-fulfillment of these criteria is an indication of deterioration of reagents or an error in the test procedure and the assay must be repeated.
General Description
Scrub Typhus is an infectious disease that is caused by Orientia tsutsugamushi (formerly Rickettsia), a tiny parasite about the size of bacteria that belongs to the family Rickettsiaceae. A bite from the larval trombiculid mite, a parasite of rodents, will transmit the disease. An ulcer of the skin is characteristic of a bite from a trombiculid mite, followed by symptoms including fever, a spotted rash on the torso, and swelling of the lymph glands. Scrub typhus generally occurs after exposure to areas with secondary (scrub) vegetation, which is where its name is derived from. However, the disease can also be prevalent in sandy, mountainous, and tropical areas. Scrub Typhus is a world wide illness, but particular to South East Asia and the Western Pacific. It accounts for approximately 20% of fever in some regions in South East Asia, where it is endemic. Illness lasts for a period of 10 to 12 days after the initial bite. With therapy, the fever will break within 36 hours, but if left untreated, complications or death may occur.


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