The test can be used to detect SVNV in infected plan tissues.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant leaf tissues diluted at 1:270.
General Description
Soybean vein necrosis virus (SVNV, previously: Soybean vein necrosis associated virus SVNaV) is a plant pathogenic virus of soybeans (Glycine max). SVNV is a relatively new virus, which was discovered in Tennessee in 2008 and has recently been found in many US states from the Southeast and East coast to some western states including CA. This pathogen initially causes intraveinal chlorosis (yellowing) in leaves. This chlorosis then spreads throughout the leaf and eventually these chlorotic areas can become necrotic. It is a member of the family Bunyaviridae and genus Tospovirus, which is the only genus within this virus family that infects plants. Like other members of Bunyaviridae, this virus is enveloped and has a negative sense single-stranded RNA (-ssRNA) genome composed of three genomic segments (S, M, and L). It encodes proteins on the M and S segments in an ambisense manner.
Standard Curve
Citations
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