SEK1/MKK4 ELISA Kit

Sample

cultured cells

Species Reactivity

Human, Mouse, Rat

Intended Use

The SEK1/MKK4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SEK1/MKK4 protein expression profile in cells. The kit can be used for measuring the relative amounts of SEK1/MKK4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SEK1/MKK4.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 1 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
: 6. 100x Anti-SEK1/MKK4 Antibody (Rabbit Polyclonal) 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
16. Adhesive Plate Seals: 4 Seals

Storage

4°C/6 Months