Sulfadiazine ELISA Kit (DEIANJ09)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
tissue, serum, honey, milk
Species Reactivity
N/A
Intended Use
The Sulfadiazine(SDZ) Kit is for the quantitative detection of Sulfadiazine (SDZ) concentration in tissue, serum, honey, milk.
Storage
Store at 2 - 8°C.

【SAMPLE STORAGE】
1. Untreated samples are stored at frozen environment.
2. Prepared sample can be stable at 2-8°C for 1 week.
Precision
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Detection Range
0.5 ppb - 40.5 ppb
Detection Limit
Tissue, Honey 0.5 ppb
Serum 2 ppb
Milk 10 ppb
Sensitivity
0.5 ppb
General Description
Sulfadiazine (SDZ), one kind of broad-spectrum antibiotic, is effective for animal production for its excellent antibacterial and pharmacokinetic properties. It has been widely used in different lines of poultry, cattle, agriculture and beekeeping. However, Sulfadiazine is prohibited in most countries as its residue may cause serious side effects, such as hematotoxic. Therefore, it is possible that sulfadiazine residues, after use in illegal practice, may lead to a risk for consumers.

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References


Incorporation of Silver Sulfadiazine into An Electrospun Composite of Polycaprolactone as An Antibacterial Scaffold for Wound Healing in Rats

CELL JOURNAL

Authors: Nejaddehbashi, Fereshteh; Hashemitabar, Mahmoud; Bayati, Vahid; Moghimipour, Eskandar; Movaffagh, Jabraeel; Orazizadeh, Mahmoud; Abbaspour, Mohammadreza

Objective: Fabrication of an antibiotic-loaded scaffold with controlled release properties for wound dressing is one of tissue engineering challenges. The aim of this study was to evaluate the wound-healing effectiveness of 500-mu m thick polycaprolactone (PCL) nanofibrous mat containing silver sulfadiazine (SSD) as an antibacterial agent. Materials and Methods: In this experimental study, an electrospun membrane of PCL nanofibrous mat containing 0.3% weight SSD with 500 mu m thickness, was prepared. Morphological and thermomechanical characteristics of nanofibers were evaluated. Drug content and drug release properties as well as the surface hydrophobicity of the nanofibrous membrane were determined. Antimicrobial properties and cellular viability of the scaffold were also examined. A full thickness wound of 400 mm(2) was created in rats, to evaluate the wound-healing effects of PCL/SSD blend in comparison with PCL and vaseline gas used as the control group. Results: SSD at a concentration of 0.3% improved physicochemical properties of PCL. This concentration of SSD did not inhibit the attachment of human dermal fibroblasts (HDFs) to nanofibers in vitro, but showed antibacterial activity against Gram-positive Staphylococcus aureus (ST) and Gram-negative Pseudomonas aeruginosa (PS). Overall, results showed that SSD improves characteristics of PCL nanofibrous film and improves wound-healing process in one-week earlier compared to control. Conclusion: Cytotoxicity of SSD in fabricated nanofibrous mat is a critical challenge in designing an effective wound dressing that neutralizes cellular toxicity and improves antimicrobial activity. The PCL/SSD nanofibrous membrane with 500-mu m thickness and 0.3% (w/v) SSD showed applicable characteristics as a wound dressing and it accelerated wound healing process in vivo.

Functionalization of amino terminated carbon nanotubes with isocyanates for magnetic solid phase extraction of sulfonamides from milk and their subsequent determination by liquid chromatography-high resolution mass spectrometry

FOOD CHEMISTRY

Authors: Fu, Lin; Zhou, Hao; Miao, Enming; Lu, Shiwei; Jing, Siyuan; Hu, Yufeng; Wei, Lijuan; Zhan, Jingjing; Wu, Minghuo

A simple modification method was developed for the functionalization of amino terminated carbon nanotubes (CNT-NH2) by using isocyanates as modifiers via the nucleophilic addition reaction. Two types of functionalized magnetic carbon nanotubes (MCNT) were prepared through deposition of magnetic nanoparticles on CNT-NH2 and modification with different isocyanates. p-Tolyl-functionalized MCNT (Tol-MCNT) with better adsorption performance were selected as adsorbent for magnetic solid phase extraction (MSPE), which could extract sulfonamides (SAs) from various milk samples with a enrichment factor of about 30 after optimization. By combining the MSPE with liquid chromatography-high resolution mass spectrometry (LC-HRMS), a new method was developed. Both skimmed and whole milk samples of three brands were analyzed with this method, and 4 SAs including sulfadiazine, sulfisomidine, sulfamethazine and sulfameter were detected with the concentration from unquantifiable to 72 ng/L, which were all well below the maximum residue limits in milk according to the regulations of China and EU.

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