Symmetric Dimethylarginine ELISA Kit (DEIA093J)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
EDTA plasma, serum
Species Reactivity
Human
Intended Use
The SDMA ELISA Kit is intended for the quantitative determination of symmetric dimethylarginine (SDMA) in human EDTA-plasma and serum.
Contents of Kit
1. Holder with precoated strips: 12 x 8 wells
2. Standards diluted in reaction buffer, ready to use: 6 x 1 vial
3. Controls diluted in reaction buffer, ready to use: 2 x 1 vial
4. ELISA Wash Buffer concentrate 10X: 2 x 100 mL
5. DMA antibody, lyophilized: 2 x 1 vial
6. Conjugate (peroxidase-labeled), concentrate: 1 x 120 μL
7. Conjugate stabilizing buffer, ready to use: 1 x 24 mL
8. Reaction buffer, ready to use: 1 x 15 mL
9. Derivatization reagent: 2 x 50 mg
10. Dimethylsulfoxide (DMSO): 1 x 7 mL
11. Dilution buffer after derivatization, ready to use: 1 x 28 mL
12. substrate (Tetramethylbenzidine), ready to use: 1 x 25 mL
13. ELISA stop solution, ready to use: 1 x 15 mL
Storage
Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.
Precision
Intra-assay Precision: 4.8%-7.5%
Inter-assay Precision: 6%-7%
Sensitivity
0.05 μmol/L
Standard Curve

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References


Lluch, P; Torondel, B; et al. Plasma concentrations of nitric oxide and asymmetric dimethylarginine in human alcoholic cirrhosis. JOURNAL OF HEPATOLOGY 41:55-59(2004).
Scalera, F; Kielstein, JT; et al. Erythropoietin increases asymmetric dimethylarginine in endothelial cells: Role of dimethylarginine dimethylaminohydrolase. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 16:892-898(2005).

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