Intended Use
The test can be used to detect SCMV in infected sugarcane and other cereal crops.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
General Description
SCMV is transmitted by several aphid species in a non-persistent manner and by mechanical inoculation. Seed transmission is found in maize, but not in sugarcane or sorghum. Based on host pants, there are many strains identified for SCMV, such as USA A, B, D, E, F in sugarcane and rarely in Johnsongrass, Australian SCMV strains BC, Sabi in grasses and strain SC in sugarcane. Maize dwarf mosaic strain B infects maize but does not infect Johnsongrass or sugarcane. Abaca mosaic strain is found in abaca and maize. Typical symptom of SCMV infection is mosaic. SCMV occurs worldwide, but does not infect wheat, barley, rye or rice naturally.
Citations
Publication ()
Have you cited DEIAPV266 in a publication?
Let us know and earn a reward for your research.
Origins and Distribution of Panicum Mosaic Virus and Sugarcane Mosaic Virus on Stenotaphrum secundatum in Australia
Nga T Tran, Ai Chin Teo, Alistair R McTaggart Paul R Campbell, Denis M Persley, John E Thomas, Andrew D W Geering
Applications: ELISA
Reactive species: Unspecified reactive species
"Abstract: Stenotaphrum secundatum is a premium turf grass in warm temperate and subtropical regions of the world and is the most important turf species in Australia based on the value of its production. A new disease called buffalo grass yellows (BGY) has become a problem on turf farms in Australia. We surveyed turf farms in New South Wales, Queensland, and Western Australia to determine whether panicum mosaic virus (PMV) and sugarcane mosaic virus (SCMV) were associated with BGY. PMV was only found on three farms, two located in the Hawkesbury Valley near Sydney and a third at Echuca, about 800 km to the southwest of the former location. SCMV was more prevalent, present in all major cultivars and states surveyed. We analyzed phylogenetic relationships for SCMV and found that isolates infecting S. secundatum in Australia belonged to three clades. The first included Australian isolates typical of the population of viruses circulating in Digitaria didactyla. The second included a single New South Wales isolate from S. secundatum 'SS100' that grouped with otherwise American isolates of SCMV recorded in S. secundatum and Saccharum officinale from Florida and Zea mays from Ohio. Finally, an isolate of SCMV from S. secundatum originating from a turf farm in South East Queensland grouped with viruses mostly infecting maize; this record is potentially the first maize-adapted strain of SCMV in Australia. Our study sheds light on the etiology of the BGY disease syndrome and invasion history of PMV and SCMV in Australia."
Article snippet: Tests for PMV and SCMV were done on each sample using commercially available double-antibody sandwich ELISA kits (catalog numbers DEIAPV200 and DEIAPV266; Creative Diagnostics, Shirley, NY) following the manufacturer's instructions, except that reaction volumes were reduced to 50μl.
COA
Loading ......
