SARS Spike protein (aa 1 - 1190) [His] (DAG2408)

SARS Spike protein (aa 1 - 1190) [His], recombinant protein from HEK 293 cells

Datasheet COA SDS
Product Overview
6xHis tagged Spike protein (amino acid 1-1190) (SARS-CoV) (Beijing02) (Genebank No.AY278487)
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
comprises 665 amino acids and has a predicted molecular mass of 142.8 kDa. As a result of high glycosylation, the apparent molecular mass of the protein is approximately 190-200 kDa in SDS-PAGE under reducing conditions
Procedure
None
Purity
≥ 95%
Format
Liquid
Concentration
1 mg/mL
Size
100 ug
Buffer
PBS containing 0.1% BSA and 25% glycerol.
Preservative
None
Storage
Store at 4°C; DO NOT FREEZE; stable for 1 year from the date of shipment.
Stability
Samples are stable for up to twelve months from date of receipt at -70 ℃
Introduction
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. The E.Coli derived 38 kDa mosaic protein contains the N-terminal section of the Spike protein.

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References

Havenga, MJE; Holterman, L; et al. Serum-free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivity. BIOTECHNOLOGY AND BIOENGINEERING 100:273-283(2008).
Yang, Y; Deng, Y; et al. The Amino Acids 736-761 of the MERS-CoV Spike Protein Induce Neutralizing Antibodies: Implications for the Development of Vaccines and Antiviral Agents. VIRAL IMMUNOLOGY 27:543-550(2014).

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