SARS Spike S1 Subunit Protein [His] (DAG-H10342)

SARS Spike Protein [His], recombinant protein from Baculovirus

Product Overview
A DNA sequence encoding the S1 subunit of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Met1-Arg667) was expressed with a C-terminal polyhistidine tag.
Nature
Recombinant
Predicted N terminal
Ser 14
Tag/Conjugate
His
Molecular Weight
The recombinant S1 subunit of human SARS coronavirus (isolate:WH20) spike comprises 665 amino acids and has a predicted molecular mass of 74.4 kDa. The apparent molecular mass of the protein is approximately 85.8 kDa in SDS-PAGE under reducing conditions
Endotoxin
< 1 .0 EU per μg of the protein as determined by the LAL method
Procedure
None
Purity
> 90 % as determined by SDS-PAGE
Format
Lyophilized
Concentration
Specific concentrations are included in the hardcopy of COA.
Size
50 ug; 100 ug
Buffer
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Preservative
None
Storage
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability
Samples are stable for up to twelve months from date of receipt at -70 ℃
Introduction
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. The E.Coli derived 38 kDa mosaic protein contains the N-terminal section of the Spike protein.

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References


Ying, TL; Du, LY; et al. Exceptionally Potent Neutralization of Middle East Respiratory Syndrome Coronavirus by Human Monoclonal Antibodies. JOURNAL OF VIROLOGY 88:7796-7805(2014).
Zhong, L; Haynes, L; et al. Antibody-mediated synergy and interference in the neutralization of SARS-CoV at an epitope cluster on the spike protein. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 390:1056-1060(2009).

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