SARS Spike Protein (Receptor Binding Domain) [Fc] (DAG-H10344)

SARS Spike Protein [Fc], recombinant protein from Baculovirus

Product Overview
A DNA sequence encoding the receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with the Fc region of rabbit IgG at the C-terminus.
Nature
Recombinant
Predicted N terminal
Arg 306
Tag/Conjugate
Fc
Molecular Weight
The recombinant receptor binding domain (RBD) of human SARScoronavirus (isolate:WH20) spike comprises 446 amino acids and has a predicted molecular mass of 50.2 kDa. The apparent molecular mass of the protein is approximately 78.9 and 54.3 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1 .0 EU per μg of the protein as determined by the LAL method
Procedure
None
Purity
(36.8+ 56.0) % as determined by SDS-PAGE
Format
Lyophilized
Concentration
Specific concentrations are included in the hardcopy of COA.
Size
50 ug; 100 ug
Buffer
Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 8.0.
Preservative
None
Storage
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability
Samples are stable for up to twelve months from date of receipt at -70 ℃
Introduction
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. The E.Coli derived 38 kDa mosaic protein contains the N-terminal section of the Spike protein.

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References


Sui, JH; Aird, DR; et al. Broadening of Neutralization Activity to Directly Block a Dominant Antibody-Driven SARS-Coronavirus Evolution Pathway. PLOS PATHOGENS 4:-(2008).
Lip, KM; Shen, S; et al. Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus. JOURNAL OF VIROLOGY 80:941-950(2006).

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