S100A8/A9 (MRP8/14, Calprotectin) ELISA Kit

Name: S100A8/A9 (MRP8/14, Calprotectin) ELISA Kit
SKU: DEIA063J
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Key features and details

This assay is an enzyme immunoassay intended for the quantitative determination of calprotectin (MRP 8/14, S100A8/A9) in serum and plasma. For research use only.

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

sELISA

Intended Use

This assay is an enzyme immunoassay intended for the quantitative determination of calprotectin (MRP 8/14, S100A8/A9) in serum and plasma. For research use only.

Contents of Kit

1. PLATE Microtiter plate, pre-coated, 12 x 8 wells
2. WASHBUF Wash buffer concentrate, 10x, 2 x 100 ml
3. SAMPLEBUF Sample dilution buffer, ready-to-use, 1 x 100 ml
4. STD Calprotectin standards, ready-to-use, (0; 3,9; 15,6; 62,5; 250 ng/ml), 1 x 5 vials
5. CTRL 1 Control, ready-to-use, (see specification for range), 1 x 1 vial
6. CTRL 2 Control, ready-to-use, (see specification for range), 1 x 1 vial
7. CONJ Conjugate, ready-to-use, 1 x 15 ml
8. SUB Substrate (tetramethylbenzidine), ready-to-use, 1 x 15 ml
9. STOP Stop solution, ready-to-use, 1 x 15 ml

Storage

2-8°C

Sensitivity

Limit of blank, LoB 0.522 ng/ml
Limit of detection, LoD 0.789 ng/ml
Limit of quantitation, LoQ 0.897 ng/ml

General Description

Calprotectin is a calcium-binding protein secreted predominantly by neutrophils and monocytes. The heterocomplex consists of the two proteins, S100A8 (calgranulin A) and S100A9 (calgranulin B), also designated as MRP8 and MRP14, respectively. Expression of S100A8 and S100A9 in epithelial tissues was first described in context with squamous epithelia and with murine and human wound repair. More recently, an association of S100 protein expression with adenocarcinomas in humans has emerged. The genes S100A8 and S100A9 are located in a gene cluster on chromosome 1q21, a region in which several rearrangements that occur during tumor devel- opment have been observed.

Reconstitution And Storage

1. To run the assay more than once, ensure that reagents are stored at the condi- tions stated on the label. Prepare only the appropriate amount necessary for each run. The kit can be used up to 4 times within the expiry date stated on the label.
2. Preparation of the wash buffer: The wash buffer concentrate (WASHBUF) has to be diluted with ultrapure water 1:10 before use (100 ml WASHBUF + 900 ml ultrapure water), mix well. Crystals could occur due to high salt con- centration in the concentrate. Before dilution, the crystals have to be redis- solved at room temperature or in a water bath at 37°C. The WASHBUF is stable at 2–8°C until the expiry date stated on the label. Wash buffer (1:10 diluted WASHBUF) can be stored in a closed flask at 2–8°C for 1 month.
3. All other test reagents are ready-to-use. Test reagents are stable until the ex- piry date (see label) when stored at 2–8°C.

Citations

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Documents

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QA & Reviews

Customer Reviews

References

Inflammatory mediators ATP and S100A12 activate the NLRP3 inflammasome to induce MUC5AC production in airway epithelial cells

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Authors: Kim, Karam; Kim, Hye Jeong; Binas, Bert; Kang, Jin Hyun; Chung, Il Yup

Abstract

Danger-associated molecular patterns (DAMPS) play a proinflammatory role in the pathogenesis of airway obstructive diseases such as severe asthma and chronic obstructive pulmonary disease. The NLRP3 inflammasome is a cytosolic multiprotein platform that activates the caspase-1 pathway in response to inflammatory stimuli such as DAMPs. ATP and S100 proteins are newly identified DAMPs that accumulate in inflamed airways. We previously demonstrated that S100A8, S100A9, and S100A12 induce production and secretion of MUC5AC, a major mucin in the conducting airway mucosa. The purpose of this study was to determine the involvement of NLRP3 inflammasome in, and the contribution of ATP to, 5100 protein-induced MUC5AC production by NCI-H292 mucoepidermoid carcinoma cells. Stimulation with either S100A12 or ATP led to MUC5AC production at comparable levels. Simultaneous treatment with both stimuli resulted in additive increases in NLRP3, active caspase-1, IL-1 beta, NLRP3/caspase-1 colocalization, and MUC5AC. NLRP3 siRNA or inhibitors of NF-kappa B, NLRP3 inflammasome oligomerization, or caspase-1 nearly completely inhibited ATP- and S100A12-mediated MUC5AC production. Furthermore, S100A12-as well as ATP-mediated MUC5AC production was almost equally blunted by both nonspecific and specific antagonists of the purinergic receptor P2X7, a principal receptor mediating NLRP3 inflammasome activation by ATP. Thus, these two danger signals contribute to MUC5AC production in airway epithelial cells through overlapping signaling pathways for NLRP3 inflammasome activation. (C) 2018 Elsevier Inc. All rights reserved.

Expression and clinical significance of S100 family genes in patients with melanoma

MELANOMA RESEARCH

Authors: Xiong, Ting-feng; Pan, Fu-qiang; Li, Dong

Abstract

Genes in the S100 family are abnormally expressed in a variety of tumor cells and are associated with clinical pathology, but their prognostic value in melanoma patients has not yet been fully elucidated. In this study, we extracted and profiled S100 family mRNA expression data and corresponding clinical data from the Gene Expression Omnibus database to analyze how expression of these genes correlates with clinical pathology. Compared with normal skin, S100A1, S100A13, and S100B were expressed at significantly higher levels in melanoma samples. S100A2, S100A7, S100A8, S100A9, S100A10, S100A11, and S100P were all highly expressed in primary melanoma samples but were expressed at low levels in metastatic melanoma, and all of these genes were strongly correlated with each other (P<0.001). We found the expression of these S100 family genes to be significantly correlated with both lymphatic and distant melanoma metastasis, as well as with American Joint Committee on Cancer grade but not with Clark's grade, age, or sex. This suggests that expression of these genes may be related to the degree of tumor invasion. Although further validation through basic and clinical trials is needed, our results suggest that the S100 family genes have the potential to play an important role in the diagnosis of melanoma. S100 expression may be related to tumor invasion and may facilitate the early diagnosis of melanoma, allowing for a more accurate prognosis. Targeted S100 therapies are also potentially viable strategies in the context of melanoma.