Rib-p-Ab ELISA Kit

Sample

serum

Species Reactivity

Human

Intended Use

Rib-P-Ab ELISA is a solid phase enzyme immunoassay employing native human ribosomal P-proteins P0, P1 and P2 isolated from eukaryotic cell line for the quantitative and qualitative detection of antibodies against ribosomal P-proteins (rib-P) in human serum.
The specificity of anti-rib-P antibodies is restricted to a common antigenic determinant located on the highly conserved carboxyl-terminal portion of the three P proteins. The assay is a tool in the diagnosis of systemic lupus erythematosus (SLE).

Contents of Kit

Sample Buffer (5x): 1 x 20ml
Wash Buffer (50x): 1 x 20ml
Negative Control: 1 x 1.5ml
Positive Control: 1 x 1.5ml
Cut-off Calibrator: 1 x 1.5ml
Calibrators: 6 x 1.5ml
Conjugate, IgG: 1 x 15ml
TMB Substrate: 1 x 15ml
Stop Solution: 1 x 15ml
Microtiter plate: 12 x 8 well strips

Storage

2-8°C

Precision

Detection Range

0 - 300 U/mL, cut-off 15 U/mL

Sensitivity

1.0 U/ml

General Description

Rib-P-Ab ELISA is a solid phase enzyme immunoassay employing native human ribosomal P-proteins P0, P1 and P2 isolated from eukaryotic celline for the quantitative detection of antibodies against ribosomal P-proteins (rib-P) in human serum. The specificity of anti-rib-P antibodies is restricted to a common antigenic determinant located on the highly conserved carboxyl-terminal portion of the three P proteins. The assay is a tool in the diagnosis of systemic lupus erythematosus (SLE). The ribosomal phosphoproteins P0 (~38 kDa), P1 (~ 19 kDa) and P2 (~17 kDa) are located within the 60S subunit of human ribosomes. In contrast to the majority of basic ribosomal proteins, P1 and P2 are acidic. The ribosomal proteins are associated to a pentamer with two P1/P2 heterodimers anchored to P0 by the amino terminal portion of P2. This pentamer is located in a highly accessible region on the stalk of the ribosome. Biochemical studies suggest that P1/P2 play a fundamental role in all three phases of ribosomal polypeptid synthesis (initiation, translocation, termination). Autoantibodies to ribosomal proteins are highly specific for SLE since they are not found in other autoimmune diseases or in infections. The frequency of anti-rib-P antibodies is 10-20% in randomly selected SLE patients. Anti-rib-P antibodies are detected more frequently in lupus patients with severe psychiatric manifestations. In addition, other organ involvement including renal and hepatic disease might be correlated with the presence of anti-rib-P.

Standard Curve