HIV Reverse Transcriptase (DAG1875)

HIV Reverse Transcriptase, recombinant protein from E. coli Datasheet

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Product Overview
Solution.
Nature
Recombinant
Tag/Conjugate
Unconjugated
Format
Liquid
Buffer
In 10 mM potassium phosphate, pH 7.4, 1 mM DTT and 20% glycerol
Storage
2-8°C short term, -20°C long term
Introduction
The HIV reverse transcriptase has ribonuclease activity that degrades the viral RNA during the synthesis of cDNA, as well as DNA-dependent DNA polymerase activity that copies the sense cDNA strand into an antisense DNA to form a double-stranded viral DNA intermediate (vDNA).
Antigen Description
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.

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