Recombinant Yeast ATP-Sulfurylase/MET3 antigen, was expressed in E. coli. Pro2-Phe511, with N-terminal Met and 6-His tag (Accession # P08536)
> 95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie. Blue stain at 5 μg per lane.
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Assay Buffer: 25 mM Tris, 1 mM MgCl2, 20 mM Na2SO4, pH 8.0
2-8°C short term, -20°C long term
In enzymology, a sulfate adenylyltransferase (EC 18.104.22.168) is an enzyme that catalyzes the chemical reaction: ATP + sulfate diphosphate + adenylyl sulfate. Thus, the two substrates of this enzyme are ATP and sulfate, whereas its two products are diphosphate and adenylyl sulfate. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). Some sulfate adenylyltransferases are part of a bifunctional polypeptide chain associated with adenosyl phosphosulfate (APS) kinase. Both enzymes are required for PAPS (phosphoadenosine-phosphosulfate) synthesis from inorganic sulfate.
Sulfur metabolism is essential to all organisms. Inorganic sulfate is sequentially activated by ATP Sulfurylase and adenosine 5′-phosphosulfate kinase (APSK). ATP Sulfurylase converts ATP to adenosine-5'-phosphosulfate (APS) and generates pyrophosphate. APSK subsequently phosphorylates APS at the 3' site to generate 3'-phosphoadenosine-5'-phosphosulfate (PAPS). PAPS is the sulfur donor required for all sulfotransferase reactions in humans and is also the sulfur source for synthesis of cysteine, methionine and glutathione. The ATP Sulfurylase reaction is highly unfavorable energetically with the standard free-energy change of approximately +46 kJ/mol. To drive the reaction forward, APSK and pyrophosphatase are generally added into the reaction simultaneously to remove the products.
Sulfate adenylyltransferase; ATP:sulfate adenylyltransferase; ATP-sulfurylase; adenosine-5'-triphosphate sulfurylase; adenosinetriphosphate sulfurylase; adenylylsulfate pyrophosphorylase ATP sulfurylase; sulfurylase; Yeast MET3 protein